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学科主题: Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology
题名: Genotyping of Vibrio alginolyticus isolates from Daya Bay by infrequent-restriction-site PCR and pulsed-field gel electrophoresis
作者: Ren, C ; Hu, C ; Luo, P ; Chen, C ; Jiang, X ; Wang, Q
通讯作者: cqhu@scsio.ac.cn
关键词: Vibrio alginolyticus ; Genotyping ; Infrequent-restriction-site PCR ; Pulsed-field gel electrophoresis
刊名: MOLECULAR AND CELLULAR PROBES
发表日期: 2008
卷: 22, 期:4, 页:267-271
收录类别: sci
部门归属: [Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Vibrio alginolyticus is a serious bacterial pathogen that hampered the whole industry of fish farming in Guangdong, China. In order to facilitate epidemiologic studies and improve the control of disease, we developed a highly efficient method of genotyping for V. alginolyticus using infrequent-restriction-site PCR (IRS-PCR) technology. With the enzyme combination of NotI-HhaI, optimized, unique, and easy-to-interpret patterns were generated. Forty-five V. alginolyticus isolates from aquatic animals and the marine environment in Daya Bay in Guangdong were fingerprinted by IRS-PCR and pulsed-field gel electrophoresis (PFGE). The reproducibility of the IRS-PCR method was high (100%) in conformity with PFGE. When primer PN-T or PN-G was used, IRS-PCR obtained the identical clustering as PFGE, producing 24 different patterns, respectively. Moreover, IRS-PCR presented a high discriminatory power (D = 0.953) identical to that of PFGE. In conclusion, the IRS-PCR fingerprinting method using NotI-HhaI can provide a potentially useful tool for epidemiologic investigations of V. alginolyticus isolates. (C) 2008 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000264314800011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4928
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Ren, C; Hu, C; Luo, P; Chen, C; Jiang, X; Wang, Q.Genotyping of Vibrio alginolyticus isolates from Daya Bay by infrequent-restriction-site PCR and pulsed-field gel electrophoresis,MOLECULAR AND CELLULAR PROBES,2008,22(4):267-271
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文件名: Genotyping of Vibrio alginolyticus isolates from Daya Bay by infrequent-restriction-site PCR and pulsed-field gel electrophoresis .pdf
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