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学科主题: Fisheries; Veterinary Sciences
题名: Vibrio alginolyticus gyrB sequence analysis and gyrB-targeted PCR identification in environmental isolates
作者: Luo, P ; Hu, CQ
通讯作者: cqhu@scsio.ac.cn
关键词: gyrB ; Vibrio alginolyticus ; PCR identification
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2008
卷: 82, 期:3, 页:209-216
收录类别: sci
部门归属: [Luo, Peng; Hu, Chaoqun] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: gyrB fragments (about 1.2 kb) of 9 Vibrio alginolyticus strains were sequenced, and their phylogenetic relationship with other closely related Vibrio species was analyzed. All the V alginolyticus strains grouped into one strongly supported cluster in the phylogenetic tree. There were 54 base variations among the 1167 bp mutual gyrB regions of 11 V alginolyticus strains; all the V alginolyticus strains shared the same amino acid sequence except V alginolyticus ATCC 17749. Based on the gyrB sequences, we designed 2 primers for specific PCR identification of V alginolyticus. Fifty-two bacterial strains from 12 genera were used to test the PCR specificity, and only V alginolyticus strains produced the predicted 568 bp amplification fragment. In addition, PCR screening of 50 randomly selected environmental strains, grown on thiosulfate citrate bile salts-sucrose (TCBS) medium, gave rise to a positive amplification result for V alginolyticus from 37 of them. To further confirm accuracy of PCR identification, biochemical identification of the 50 strains was carried out. Strains giving positive PCR amplification were biochemically identified as V alginolyticus, while strains that gave negative results were biochemically identified as other Vibrio or non-Vibrio species. Using the basic local alignment search tool (BLAST), gyrB sequences obtained from 2 randomly selected strains (YJ0666 and YJ16713) of the 37 PCR-positive strains showed highest identity values with V. alginolyticus strains (>96%). Thus, our results demonstrated that gyrB is a good marker for molecular identification of V alginolyticus, and a gyrB-based PCR method was successfully developed.
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WOS记录号: WOS:000262839900005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4932
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Luo, P; Hu, CQ.Vibrio alginolyticus gyrB sequence analysis and gyrB-targeted PCR identification in environmental isolates,DISEASES OF AQUATIC ORGANISMS,2008,82(3):209-216
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