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学科主题: Agronomy; Plant Sciences; Horticulture
题名: Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp.
作者: Xie, LW ; Jiang, SM ; Zhu, HH ; Sun, W ; Ouyang, YC ; Dai, SK ; Li, X
通讯作者: lixiang@scsio.ac.cn
关键词: Sclerotinia sclerotiorum ; Roridin A ; Roridin D ; Myrothecium sp. ; Axinella sp. ; Activity tracking method
刊名: EUROPEAN JOURNAL OF PLANT PATHOLOGY
发表日期: 2008
卷: 122, 期:4, 页:571-578
收录类别: sci
部门归属: [Xie, Lian Wu; Jiang, Shu Mei; Sun, Wei; Ouyang, Yong Chang; Dai, Shi Kun; Li, Xiang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Marine Biorecourses Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Xie, Lian Wu; Sun, Wei; Ouyang, Yong Chang; Dai, Shi Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhu, Hong Hui] Guangdong Acad Sci, Guangdong Inst Microbiol, Guangzhou 510070, Guangdong, Peoples R China
项目归属: LMB
摘要: Sclerotinia sclerotiorum is a worldwide ascomycete fungal plant pathogen, which causes enormous yield losses on major economic crops such as crucifers, grain legumes and several other plant families. The objective of this research was to isolate and characterise some bioactive products from cultures of fungi associated with the marine sponge Axinella sp. In total, nine fungal isolates were obtained from the marine sponge Axinella sp. collected from the South China Sea. A group of test strains, including two G(+) strains (Bacillus subtilis and Staphylococcus aureus), two G(-) strains (Escherichia coli and Pseudomonas aeruginosa) and three fungi including two plant pathogenic fungi Sclerotinia sclerotiorum and Magnaporthe grisea and Saccharomyces cerevisiae, were employed as the indicator organisms for bioactivity screening. Using antagonistic tests and bioactive screening of the ethyl acetate (EtOAc) extracts of the corresponding cultures, fungal isolate JS9 showed the stronger efficacy against the test indicator strains, especially the indicator fungal pathogens. Isolate JS9 was further identified as Myrothecium sp. by a combination of morphological features and 18S rDNA BLAST on GenBank. Two macrocyclic trichothecenes, roridin A (compound 1) and roridin D (compound 2) were purified by tracking the activity of the EtOAc extract fractions and characterised with spectral analyses including MS, H-1-NMR, C-13-NMR and disortionless enhancement by polarization transfer (DEPT). In vitro antifungal tests showed that the two macrocyclic trichothecenes were bioactive against S. cerevisiae, M. grisea and S. sclerotiorum with minimal inhibitory concentrations of 31.25, 125 and 31.25 mu g ml(-1) for roridin A, and 62.5, 250 and 31.25 mu g ml(-1) for roridin D, respectively. The present investigation demonstrated that two antifungal trichothecenes including roridin A and roridin D produced by the fungus Myrothecium sp. isolated from the marine sponge Axinella sp. could be potential inhibitors against the plant pathogen S. sclerotiorum.
原文出处: 查看原文
WOS记录号: WOS:000260540400012
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4948
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Xie, LW; Jiang, SM; Zhu, HH; Sun, W; Ouyang, YC; Dai, SK; Li, X.Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp.,EUROPEAN JOURNAL OF PLANT PATHOLOGY,2008,122(4):571-578
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