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Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp.
Xie, LW; Jiang, SM; Zhu, HH; Sun, W; Ouyang, YC; Dai, SK; Li, X; lixiang@scsio.ac.cn
2008
Source PublicationEUROPEAN JOURNAL OF PLANT PATHOLOGY
ISSN0929-1873
Volume122Issue:4Pages:571-578
AbstractSclerotinia sclerotiorum is a worldwide ascomycete fungal plant pathogen, which causes enormous yield losses on major economic crops such as crucifers, grain legumes and several other plant families. The objective of this research was to isolate and characterise some bioactive products from cultures of fungi associated with the marine sponge Axinella sp. In total, nine fungal isolates were obtained from the marine sponge Axinella sp. collected from the South China Sea. A group of test strains, including two G(+) strains (Bacillus subtilis and Staphylococcus aureus), two G(-) strains (Escherichia coli and Pseudomonas aeruginosa) and three fungi including two plant pathogenic fungi Sclerotinia sclerotiorum and Magnaporthe grisea and Saccharomyces cerevisiae, were employed as the indicator organisms for bioactivity screening. Using antagonistic tests and bioactive screening of the ethyl acetate (EtOAc) extracts of the corresponding cultures, fungal isolate JS9 showed the stronger efficacy against the test indicator strains, especially the indicator fungal pathogens. Isolate JS9 was further identified as Myrothecium sp. by a combination of morphological features and 18S rDNA BLAST on GenBank. Two macrocyclic trichothecenes, roridin A (compound 1) and roridin D (compound 2) were purified by tracking the activity of the EtOAc extract fractions and characterised with spectral analyses including MS, H-1-NMR, C-13-NMR and disortionless enhancement by polarization transfer (DEPT). In vitro antifungal tests showed that the two macrocyclic trichothecenes were bioactive against S. cerevisiae, M. grisea and S. sclerotiorum with minimal inhibitory concentrations of 31.25, 125 and 31.25 mu g ml(-1) for roridin A, and 62.5, 250 and 31.25 mu g ml(-1) for roridin D, respectively. The present investigation demonstrated that two antifungal trichothecenes including roridin A and roridin D produced by the fungus Myrothecium sp. isolated from the marine sponge Axinella sp. could be potential inhibitors against the plant pathogen S. sclerotiorum.
Department[Xie, Lian Wu; Jiang, Shu Mei; Sun, Wei; Ouyang, Yong Chang; Dai, Shi Kun; Li, Xiang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Marine Biorecourses Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Xie, Lian Wu; Sun, Wei; Ouyang, Yong Chang; Dai, Shi Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhu, Hong Hui] Guangdong Acad Sci, Guangdong Inst Microbiol, Guangzhou 510070, Guangdong, Peoples R China
KeywordSclerotinia Sclerotiorum Roridin a Roridin d Myrothecium Sp. Axinella Sp. Activity Tracking Method
Subject AreaAgronomy ; Plant Sciences ; Horticulture
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Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000260540400012
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/4948
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorlixiang@scsio.ac.cn
Recommended Citation
GB/T 7714
Xie, LW,Jiang, SM,Zhu, HH,et al. Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp.[J]. EUROPEAN JOURNAL OF PLANT PATHOLOGY,2008,122(4):571-578.
APA Xie, LW.,Jiang, SM.,Zhu, HH.,Sun, W.,Ouyang, YC.,...&lixiang@scsio.ac.cn.(2008).Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp..EUROPEAN JOURNAL OF PLANT PATHOLOGY,122(4),571-578.
MLA Xie, LW,et al."Potential inhibitors against Sclerotinia sclerotiorum, produced by the fungus Myrothecium sp associated with the marine sponge Axinella sp.".EUROPEAN JOURNAL OF PLANT PATHOLOGY 122.4(2008):571-578.
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