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学科主题: Endocrinology & Metabolism
题名: Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change
作者: Zhang, Y ; Zhang, WM ; Yang, HY ; Zhou, WL ; Hu, CQ ; Zhang, LH
通讯作者: zhlih@mail.sysu.eeu.cn
刊名: JOURNAL OF ENDOCRINOLOGY
发表日期: 2008
卷: 199, 期:2, 页:317-331
收录类别: sci
部门归属: [Zhang, Yang; Zhang, Weimin; Yang, Huiyi; Zhou, Wenliang; Zhang, Lihong] Sun Yat Sen Univ, Sch Life Sci, Dept Biol, Guangzhou 510275, Guangdong, Peoples R China; [Zhang, Weimin] Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Hu, Chaoqun] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Previously, the ricefield eel (Monopterus albus) was speculated to have only one cytochrome p450 aromatase gene. In this study, however, the cDNAs encoding two distinct cytochrome p450 aromatases, cyp19a1a and cyp19a1b, were isolated. The genomic organizations of both cyp19 genes were conserved when compared with other teleosts. Northern blot detected In abundant expression of cyp19a1a in the ovary, and cyp19a1b in the hypothalamus. RT-PCR coupled with Southern blot showed that cyp19a1a was expressed predominantly in the gonads of both sexes, with higher levels in the ovary than testis, while cyp19a1b was expressed in all the issues examined in the male, but only in the brain and pituitary in the female. The levels of cyp19a1a mRNA in the ovary were increased significantly during vitellogenesis, but decreased significantly at mature stage. The levels of cyp19a1b mKNA in the brain and pituitary did not vary significantly during vitellogenesis. As ovarian development shifted from vitellogenesis to maturation, the levels of cyp19a1b mRNA was decreased significantly in the brain, but increased significantly in the pituitary. During natural sex change from female to male, the levels of cyp19a1a mRNA in the gonad were significantly decreased. The levels of cyp19a1b mRNA in the hypothalamus were significantly increased at the early intersexual phase, whereas the expression levels in the pituitary were sigificantly decreased at the intersexual phases. Taken together, these results showed a novel sexual dimorphism of cyp19a1b mRNA tissue distribution, and both CYP19 genes were associated with the ovarian development and natural sex change of the ricefield eel.
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WOS记录号: WOS:000260768600017
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4956
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Zhang, Y; Zhang, WM; Yang, HY; Zhou, WL; Hu, CQ; Zhang, LH.Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change,JOURNAL OF ENDOCRINOLOGY,2008,199(2):317-331
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文件名: Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus mRNA expression during ovarian development and sex change .pdf
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