Geographic distribution of Karlodinium veneficum in the US east coast as detected by ITS-ferredoxin real-time PCR assay
Zhang, H; Litaker, W; Vandersea, MW; Tester, P; Lin, SJ; senjie.lin@uconn.edu
2008
发表期刊JOURNAL OF PLANKTON RESEARCH
ISSN0142-7873
卷号30期号:8页码:905-922
摘要Karlodinium veneficum is an ichthyotoxic dinoflagellate suspected to be responsible for massive fish kills. Because it is morphologically similar to Pfiesteria and related dinoflagellates, accurate abundance data of K. veneficum in the natural environment are difficult to obtain using light microscopy. In this study, species-specific real-time PCR assays were developed using the ferredoxin gene (KvFERR) and rDNA locus (partial internal transcribed spacer regions and 5.8S rDNA, KvITS). These assays were used to investigate the geographic and temporal distribution of K. veneficum from various estuaries and coastal regions of the Pacific and West Atlantic Oceans. In general, both genes gave similar quantitative results, detecting down to 0.1-1 K. veneficum cells mL(-1). The higher occurrence frequency and abundance of K. veneficum were observed in Neuse River, North Carolina, Narragansett Bay, Rhode Island and Chesapeake Bay, MD, USA. Most of the KvITS sequences from North Carolina to Maine were identical to that of K. veneficum strain CCMP1975 (initially isolated from Chesapeake Bay), with 1-2 nucleotide substitutions occurring in sequences from Texas and Narragansett Bay samples. This result suggests the same origin and recent separation of K. veneficum populations living in this wide geographic range. In this study, K. veneficum was more abundant at 5-15 degrees C and 4-20 psu.
部门归属[Zhang, Huan; Lin, Senjie] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA; [Litaker, Wayne; Vandersea, Mark W.; Tester, Patricia] NOAA, Natl Ocean Serv, Beaufort, NC 28516 USA; [Lin, Senjie] Chinese Acad Sci, S China Sea Inst Oceanol, LMB, Guangzhou 510301, Guangdong, Peoples R China
学科领域Marine & Freshwater Biology ; Oceanography
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收录类别sci
资助项目LMB
WOS记录号WOS:000258331300005
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4968
专题中科院海洋生物资源可持续利用重点实验室
通讯作者senjie.lin@uconn.edu
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Zhang, H,Litaker, W,Vandersea, MW,et al. Geographic distribution of Karlodinium veneficum in the US east coast as detected by ITS-ferredoxin real-time PCR assay[J]. JOURNAL OF PLANKTON RESEARCH,2008,30(8):905-922.
APA Zhang, H,Litaker, W,Vandersea, MW,Tester, P,Lin, SJ,&senjie.lin@uconn.edu.(2008).Geographic distribution of Karlodinium veneficum in the US east coast as detected by ITS-ferredoxin real-time PCR assay.JOURNAL OF PLANKTON RESEARCH,30(8),905-922.
MLA Zhang, H,et al."Geographic distribution of Karlodinium veneficum in the US east coast as detected by ITS-ferredoxin real-time PCR assay".JOURNAL OF PLANKTON RESEARCH 30.8(2008):905-922.
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