Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance
Li, MW; Lin, RQ; Song, HQ; Sani, RA; Wu, XY; Zhu, XQ; xingquanzh@scau.edu.cn
2008
发表期刊ELECTROPHORESIS
ISSN0173-0835
卷号29期号:13页码:2912-2917
摘要Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), among and within Toxocara canis, T cati, T malaysiensis, T vitulorum and Toxascaris leonina from different geographical origins was examined by a mutation-scanning approach. A portion of the cox1 gene (pcox1), a portion of the nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual ascaridoid nematodes by polymerase chain reaction and the amplicons analyzed by single-strand conformation polymorphism (SSCP). Representative samples displaying sequence variation in SSCP profiles were subjected to sequencing in order to define genetic markers for their specific identification and differentiation. While the intra-specific sequence variations within each of the five ascaridoid species were 0.2-3.7% for pcox1, 0-2.8% for pnad1 and 0-2.3% for pnad4, the inter-specific sequence differences were significantly higher, being 7.9-12.9% for pcox1, 10.7-21.1% for pnad1 and 12.9-21.7% for pnad4, respectively. Phylogenetic analyses based on the combined sequences of pcoxl, pnad1 and pnad4 revealed that the recently described species T malaysiensis was more closely related to T cati than to T canis. These findings provided mtDNA evidence for the validity of T malaysiensis and also demonstrated clearly the usefulness and attributes of the mutation-scanning sequencing approach for studying the population genetic structures of these and other nematodes of socio-economic importance.
部门归属[Li, Ming-Wei; Lin, Rui-Qing; Song, Hui-Qun; Zhu, Xing-Quan] S China Agr Univ, Coll Vet Med, Parasitol Lab, Guangzhou 510642, Guangdong, Peoples R China; [Li, Ming-Wei] Guangdong Ocean Univ, Coll Agr, Dept Vet Med, Guangzhou, Guangdong, Peoples R China; [Sani, Rehana A.] Univ Putra Malaysia, Fac Vet Med, Selangor, DE, Malaysia; [Wu, Xiang-Yun] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Beijing 100864, Guangdong, Peoples R China
关键词Genetic Variation Mitochondrial Dna (mtDna) Single-strand Conformation Polymorphism (Sscp) Toxascaris Toxocara
学科领域Biochemical Research Methods ; Chemistry, Analytical
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收录类别sci
资助项目LMB
WOS记录号WOS:000257826400019
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/4972
专题中科院海洋生物资源可持续利用重点实验室
通讯作者xingquanzh@scau.edu.cn
推荐引用方式
GB/T 7714
Li, MW,Lin, RQ,Song, HQ,et al. Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance[J]. ELECTROPHORESIS,2008,29(13):2912-2917.
APA Li, MW.,Lin, RQ.,Song, HQ.,Sani, RA.,Wu, XY.,...&xingquanzh@scau.edu.cn.(2008).Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.ELECTROPHORESIS,29(13),2912-2917.
MLA Li, MW,et al."Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance".ELECTROPHORESIS 29.13(2008):2912-2917.
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