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学科主题: Microbiology
题名: Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis
作者: Zhang, YY ; Dong, JD ; Yang, ZH ; Zhang, S ; Wang, YS
通讯作者: amy0517@gmail.com
关键词: mangrove sediments ; nitrogen-fixing bacteria ; denaturing gradient gel electrophoresis ; multivariate analysis
刊名: ARCHIVES OF MICROBIOLOGY
发表日期: 2008
卷: 190, 期:1, 页:19-28
收录类别: sci
部门归属: [Zhang, Yanying; Dong, Junde; Yang, Zhihao; Zhang, Si; Wang, Youshao] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Yanying; Dong, Junde; Yang, Zhihao; Zhang, Si] Natl Expt Stn Trop Marine Biol, Sanya 572000, Hainan Island, Peoples R China; [Zhang, Yanying; Yang, Zhihao] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
项目归属: LMB
摘要: Culture-independent PCR-denaturing gradient gel electrophoresis (DGGE) was employed to assess the composition of diazotroph species from the sediments of three mangrove ecosystem sites in Sanya, Hainan Island, China. A strategy of removing humic acids prior to DNA extraction was conducted, then total community DNA was extracted using the soil DNA kit successfully for nifH PCR amplification, which simplified the current procedure and resulted in good DGGE profiles. The results revealed a novel nitrogen-fixing bacterial profile and fundamental diazotrophic biodiversity in mangrove sediments, as reflected by the numerous bands present DGGE patterns. Canonical correspondence analysis (CCA) revealed that the sediments organic carbon concentration and available soil potassium accounted for a significant amount of the variability in the nitrogen-fixing bacterial community composition. The predominant DGGE bands were sequenced, yielding 31 different nifH sequences, which were used in phylogenetic reconstructions. Most sequences were from Proteobacteria, e.g. alpha, gamma, beta, delta-subdivisions, and characterized by sequences of members of genera Azotobacter, Desulfuromonas, Sphingomonas, Geobacter, Pseudomonas, Bradyrhizobium and Derxia. These results significantly expand our knowledge of the nitrogen-fixing bacterial diversity of the mangrove environment.
原文出处: 查看原文
WOS记录号: WOS:000256927000003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/4982
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Zhang, YY; Dong, JD; Yang, ZH; Zhang, S; Wang, YS.Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR-denaturing gradient gel electrophoresis,ARCHIVES OF MICROBIOLOGY,2008,190(1):19-28
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