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学科主题: Agriculture, Dairy & Animal Science; Genetics & Heredity
题名: Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas
作者: Wang, Y ; Ren, R ; Yu, Z
通讯作者: carlzyu@scsio.ac.cn
关键词: Crassostrea gigas ; cross-species amplification ; EST-SSR ; microsatellite
刊名: ANIMAL GENETICS
发表日期: 2008
卷: 39, 期:3, 页:287-289
收录类别: sci
部门归属: [Wang, Y.; Ren, R.; Yu, Z.] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: A set of expressed sequence tag-simple sequence repeat (EST-SSR) markers of the Pacific oyster, Crassostrea gigas, was developed through bioinformatic mining of the GenBank public database. As of June 30, 2007, a total of 5132 EST sequences from GenBank were downloaded and screened for di-, tri- and tetra-nucleotide repeats, with criteria set at a minimum of 5, 4 and 4 repeats for the three categories of SSRs respectively. Seventeen polymorphic microsatellite markers were characterized. Allele numbers ranged from 3 to 10, and the observed and expected heterozygosity values varied from 0.125 to 0.770 and from 0.113 to 0.732 respectively. Eleven loci were at Hardy-Weinberg equilibrium (HWE); the other six loci showed significant departure from HWE (P < 0.01), suggesting possible presence of null alleles. Pairwise check of linkage disequilibrium (LD) indicated that 11 of 136 pairs of loci showed significant LD (P < 0.01), likely due to HWE present in single markers. Cross-species amplification was examined for five other Crassostrea species and reasonable results were obtained, promising usefulness of these markers in oyster genetics.
原文出处: 查看原文
WOS记录号: WOS:000255475700011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5002
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wang, Y; Ren, R; Yu, Z.Bioinformatic mining of EST-SSR loci in the Pacific oyster, Crassostrea gigas,ANIMAL GENETICS,2008,39(3):287-289
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