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学科主题: Biotechnology & Applied Microbiology; Medicine, Research & Experimental
题名: Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus
作者: Zhao, JJ ; Chen, C ; Zhang, LP ; Hu, CQ
通讯作者: cqhu@scsio.ac.cn
刊名: JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
发表日期: 2009
页: -
收录类别: sci
部门归属: [Zhao, Jing-jing; Chen, Chang; Zhang, Lv-ping; Hu, Chao-qun] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhao, Jing-jing] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
项目归属: LMB
摘要: Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr). These colony variants show great differences in biofilm formation and motility. In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized. The absence of rpoX did not affect colony switching rate but did decrease biofilm formation in both the Op and the Tr variants. When challenged with hydrogen peroxide, the Delta rpoX in the Op background showed a slightly higher survival rate compared with the wild type, whereas survival was decreased in the Tr background. Deletion of rpoX in the Tr background resulted in a higher ability to resist ethanol challenges and to survive hyperosmolarity challenges, and in the Op background the opposite phenotype was observed. This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus. Copyright (C) 2009 Jing-jing Zhao et al.
原文出处: 查看原文
WOS记录号: WOS:000274887500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5022
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Zhao, JJ; Chen, C; Zhang, LP; Hu, CQ.Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus,JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2009,():-
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文件名: Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus.pdf
格式: Adobe PDF
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