Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus
Zhao, JJ; Chen, C; Zhang, LP; Hu, CQ; cqhu@scsio.ac.cn
2009
发表期刊JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
ISSN1110-7243
页码-
摘要Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr). These colony variants show great differences in biofilm formation and motility. In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized. The absence of rpoX did not affect colony switching rate but did decrease biofilm formation in both the Op and the Tr variants. When challenged with hydrogen peroxide, the Delta rpoX in the Op background showed a slightly higher survival rate compared with the wild type, whereas survival was decreased in the Tr background. Deletion of rpoX in the Tr background resulted in a higher ability to resist ethanol challenges and to survive hyperosmolarity challenges, and in the Op background the opposite phenotype was observed. This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus. Copyright (C) 2009 Jing-jing Zhao et al.
部门归属[Zhao, Jing-jing; Chen, Chang; Zhang, Lv-ping; Hu, Chao-qun] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhao, Jing-jing] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
学科领域Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
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收录类别sci
资助项目LMB
WOS记录号WOS:000274887500001
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5022
专题中科院海洋生物资源可持续利用重点实验室
通讯作者cqhu@scsio.ac.cn
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Zhao, JJ,Chen, C,Zhang, LP,et al. Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2009:-.
APA Zhao, JJ,Chen, C,Zhang, LP,Hu, CQ,&cqhu@scsio.ac.cn.(2009).Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,-.
MLA Zhao, JJ,et al."Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2009):-.
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