Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis
Xia, JJ; He, XC; Yu, ZN; carlzyu@scsio.ac.cn
2009
发表期刊CONSERVATION GENETICS
ISSN1566-0621
卷号10期号:6页码:1829-1832
摘要Fourteen polymorphic microsatellite markers were isolated from the Hong Kong oyster, Crassostrea hongkongensis, with a partial genomic library enriched for tandem repeat sequences of (CA)(12), (GA)(12), (ATG)(6) and (TAGA)(4). Polymorphism of these loci was assessed in a sample of 48 wild unrelated individuals. The average allelic number of these polymorphic loci was 6.36 per locus, with a range of 4-16. The observed and expected heterozygosity varied from 0.208 to 0.729 (averaging 0.502) and from 0.193 to 0.789 (averaging 0.615), respectively. After Bonferroni correction (P > 0.0036), 11 of the 14 loci accorded with Hardy-Weinberg equilibrium, and the rest three were detected significant departure from HWE. Additionally, two loci (Ch103 and Ch104) showed significant linkage disequilibrium (P < 0.01). This is the first set of microsatellite loci developed in this species and would be useful for studies of population genetics, stock management and other relevant research in C. hongkongensis.
部门归属[Xia, Jianjun; He, Xiaocui; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Marine Bioresource Sustainable Utilizat, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China
关键词Crassostrea Hongkongensis Microsatellites Isolation Polymorphism
学科领域Biodiversity Conservation ; Genetics & Heredity
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收录类别sci
资助项目LMB
WOS记录号WOS:000272374100024
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5042
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Xia, JJ,He, XC,Yu, ZN,et al. Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis[J]. CONSERVATION GENETICS,2009,10(6):1829-1832.
APA Xia, JJ,He, XC,Yu, ZN,&carlzyu@scsio.ac.cn.(2009).Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis.CONSERVATION GENETICS,10(6),1829-1832.
MLA Xia, JJ,et al."Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis".CONSERVATION GENETICS 10.6(2009):1829-1832.
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