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Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis
Xia, JJ; He, XC; Yu, ZN; carlzyu@scsio.ac.cn
2009
Source PublicationCONSERVATION GENETICS
ISSN1566-0621
Volume10Issue:6Pages:1829-1832
AbstractFourteen polymorphic microsatellite markers were isolated from the Hong Kong oyster, Crassostrea hongkongensis, with a partial genomic library enriched for tandem repeat sequences of (CA)(12), (GA)(12), (ATG)(6) and (TAGA)(4). Polymorphism of these loci was assessed in a sample of 48 wild unrelated individuals. The average allelic number of these polymorphic loci was 6.36 per locus, with a range of 4-16. The observed and expected heterozygosity varied from 0.208 to 0.729 (averaging 0.502) and from 0.193 to 0.789 (averaging 0.615), respectively. After Bonferroni correction (P > 0.0036), 11 of the 14 loci accorded with Hardy-Weinberg equilibrium, and the rest three were detected significant departure from HWE. Additionally, two loci (Ch103 and Ch104) showed significant linkage disequilibrium (P < 0.01). This is the first set of microsatellite loci developed in this species and would be useful for studies of population genetics, stock management and other relevant research in C. hongkongensis.
Department[Xia, Jianjun; He, Xiaocui; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Marine Bioresource Sustainable Utilizat, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China
KeywordCrassostrea Hongkongensis Microsatellites Isolation Polymorphism
Subject AreaBiodiversity Conservation ; Genetics & Heredity
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Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000272374100024
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/5042
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
Xia, JJ,He, XC,Yu, ZN,et al. Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis[J]. CONSERVATION GENETICS,2009,10(6):1829-1832.
APA Xia, JJ,He, XC,Yu, ZN,&carlzyu@scsio.ac.cn.(2009).Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis.CONSERVATION GENETICS,10(6),1829-1832.
MLA Xia, JJ,et al."Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis".CONSERVATION GENETICS 10.6(2009):1829-1832.
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