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学科主题: Biodiversity Conservation; Genetics & Heredity
题名: Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis
作者: Xia, JJ ; He, XC ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Crassostrea hongkongensis ; Microsatellites ; Isolation ; Polymorphism
刊名: CONSERVATION GENETICS
发表日期: 2009
卷: 10, 期:6, 页:1829-1832
收录类别: sci
部门归属: [Xia, Jianjun; He, Xiaocui; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Marine Bioresource Sustainable Utilizat, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China
项目归属: LMB
摘要: Fourteen polymorphic microsatellite markers were isolated from the Hong Kong oyster, Crassostrea hongkongensis, with a partial genomic library enriched for tandem repeat sequences of (CA)(12), (GA)(12), (ATG)(6) and (TAGA)(4). Polymorphism of these loci was assessed in a sample of 48 wild unrelated individuals. The average allelic number of these polymorphic loci was 6.36 per locus, with a range of 4-16. The observed and expected heterozygosity varied from 0.208 to 0.729 (averaging 0.502) and from 0.193 to 0.789 (averaging 0.615), respectively. After Bonferroni correction (P > 0.0036), 11 of the 14 loci accorded with Hardy-Weinberg equilibrium, and the rest three were detected significant departure from HWE. Additionally, two loci (Ch103 and Ch104) showed significant linkage disequilibrium (P < 0.01). This is the first set of microsatellite loci developed in this species and would be useful for studies of population genetics, stock management and other relevant research in C. hongkongensis.
原文出处: 查看原文
WOS记录号: WOS:000272374100024
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5042
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Xia, JJ; He, XC; Yu, ZN.Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis,CONSERVATION GENETICS,2009,10(6):1829-1832
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文件名: Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis .pdf
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