Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of Iridoviridae
Huang, YH; Huang, XH; Liu, H; Gong, J; Ouyang, ZL; Cui, HC; Cao, JH; Zhao, YT; Wang, XJ; Jiang, YL; Qin, QW; yhh917@yahoo.com.cn; huangxh0901@yahoo.com.cn; ciqliuhong@gmail.com; gongjiezsu@yahoo.com.cn; my_yjl@yahoo.com.cn; chc1007@163.com; jianhaoc2@gmail.com; ytzhao@genetics.ac.cn; xjwang@genetics.ac.cn; szapqbxi@public.szptt.net.cn; lssqinqw@mail.sysu.edu.cn
2009
发表期刊BMC GENOMICS
ISSN1471-2164
卷号10页码:-
摘要Background: Soft-shelled turtle iridovirus (STIV) is the causative agent of severe systemic diseases in cultured soft-shelled turtles (Trionyx sinensis). To our knowledge, the only molecular information available on STIV mainly concerns the highly conserved STIV major capsid protein. The complete sequence of the STIV genome is not yet available. Therefore, determining the genome sequence of STIV and providing a detailed bioinformatic analysis of its genome content and evolution status will facilitate further understanding of the taxonomic elements of STIV and the molecular mechanisms of reptile iridovirus pathogenesis. Results: We determined the complete nucleotide sequence of the STIV genome using 454 Life Science sequencing technology. The STIV genome is 105 890 bp in length with a base composition of 55.1% G+C. Computer assisted analysis revealed that the STIV genome contains 105 potential open reading frames (ORFs), which encode polypeptides ranging from 40 to 1,294 amino acids and 20 microRNA candidates. Among the putative proteins, 20 share homology with the ancestral proteins of the nuclear and cytoplasmic large DNA viruses (NCLDVs). Comparative genomic analysis showed that STIV has the highest degree of sequence conservation and a colinear arrangement of genes with frog virus 3 (FV3), followed by Tiger frog virus (TFV), Ambystoma tigrinum virus (ATV), Singapore grouper iridovirus (SGIV), Grouper iridovirus (GIV) and other iridovirus isolates. Phylogenetic analysis based on conserved core genes and complete genome sequence of STIV with other virus genomes was performed. Moreover, analysis of the gene gain-and-loss events in the family Iridoviridae suggested that the genes encoded by iridoviruses have evolved for favoring adaptation to different natural host species. Conclusion: This study has provided the complete genome sequence of STIV. Phylogenetic analysis suggested that STIV and FV3 are strains of the same viral species belonging to the Ranavirus genus in the Iridoviridae family. Given virus-host co-evolution and the phylogenetic relationship among vertebrates from fish to reptiles, we propose that iridovirus might transmit between reptiles and amphibians and that STIV and FV3 are strains of the same viral species in the Ranavirus genus.
部门归属[Liu, Hong; Jiang, Yulin] Shenzhen Exit & Entry Inspect & Quarantine Bur, Shenzhen 518001, Peoples R China; [Huang, Youhua; Gong, Jie; Ouyang, Zhengliang; Cui, Huachun; Cao, Jianhao] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China; [Huang, Xiaohong; Qin, Qiwei] Chinese Acad Sci, Marine Biol Lab, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China; [Zhao, Yingtao; Wang, Xiujie] Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
学科领域Biotechnology & Applied Microbiology ; Genetics & Heredity
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收录类别sci
资助项目LMB
WOS记录号WOS:000266861300001
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5106
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yhh917@yahoo.com.cn; huangxh0901@yahoo.com.cn; ciqliuhong@gmail.com; gongjiezsu@yahoo.com.cn; my_yjl@yahoo.com.cn; chc1007@163.com; jianhaoc2@gmail.com; ytzhao@genetics.ac.cn; xjwang@genetics.ac.cn; szapqbxi@public.szptt.net.cn; lssqinqw@mail.sysu.edu.cn
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Huang, YH,Huang, XH,Liu, H,et al. Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of Iridoviridae[J]. BMC GENOMICS,2009,10:-.
APA Huang, YH.,Huang, XH.,Liu, H.,Gong, J.,Ouyang, ZL.,...&lssqinqw@mail.sysu.edu.cn.(2009).Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of Iridoviridae.BMC GENOMICS,10,-.
MLA Huang, YH,et al."Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of Iridoviridae".BMC GENOMICS 10(2009):-.
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