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学科主题: Biochemistry & Molecular Biology
题名: Molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from Fenneropenaeus merguiensis
作者: Mai, WJ ; Hu, CQ
通讯作者: mwj9876@yahoo.com.cn ; cqhu@scsio.ac.cn
关键词: Lysozyme C ; Fenneropenaeus merguiensis ; PCR-cloning ; Expression ; Antibacterial activity
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2009
卷: 36, 期:6, 页:1587-1595
收录类别: sci
部门归属: [Mai, Wei-jun; Hu, Chao-qun] S China Sea Inst Oceanol, Sci Acad China, Appl Marine Biol Lab, Guangzhou, Guangdong, Peoples R China
项目归属: LMB
摘要: The gene coding for lysozyme in banana prawn (Fenneropenaeus merguiensis) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. merguiensis lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 12% SDS-PAGE. Semiquantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of hemocytes, gill, gonad and lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in hemolymph challenged by Micrococcus luteus and V. alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post stimulation, respectively, and then dropped back to the original level. Bacteriolytic activity of lysozyme against different bacterial cultures was determined by solid phase as well as turbidimetric assay. Lysis was obtained against Gram positive and Gram negative bacteria with strong inhibition against shrimp pathogens V. alginolyticus and V. parahemolyticus. In addition, the study of inhibition mechanism revealed that the antibacterial activity of lysozyme was a result of bactericidal effect.
原文出处: 查看原文
WOS记录号: WOS:000266454500054
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5108
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Mai, WJ; Hu, CQ.Molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from Fenneropenaeus merguiensis,MOLECULAR BIOLOGY REPORTS,2009,36(6):1587-1595
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文件名: Molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from Fenneropenaeus merguiensis .pdf
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