中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Materials Science, Multidisciplinary; Multidisciplinary Sciences
题名: cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)
作者: Mai, WJ ; Hu, CQ
通讯作者: chqhu@scsio.ac.cn
关键词: Lysozyme C ; Litopenaeus stylirostris ; PCR-cloning ; Expression ; Antibacterial activity
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2009
卷: 19, 期:7, 页:837-844
收录类别: sci
部门归属: [Mai, Weijun; Hu, Chaoqun] Sci Acad China, S China Sea Inst Oceanol, Appl Marine Biol Lab, Guangzhou 510301, Guangdong, Peoples R China; [Mai, Weijun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
项目归属: LMB
摘要: The gene coding for lysozyme in blue shrimp (Litopenaeus stylirostris) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. merguiensi lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 15% SDS-PAGE. Semi-quantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of haemocytes, gill, gonad and the lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after the heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in haemolymph challenged by Micrococcus luteus and V. alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post-stimulation, respectively, and then dropped back to the original level. Bacteriolytic activity of the lysozyme against different bacterial cultures was determined by the solid phase and turbidimetric assays. The results demonstrated that the lysozyme we obtained was not only against Gram-positive and Gram-negative bacteria but also against shrimp pathogens V. alginolyticus and V. parahemolyticus. In addition, the study of the inhibition mechanism revealed that the antibacterial activity of the lysozyme was a result of the bactericidal effect. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000266345100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5110
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Mai, WJ; Hu, CQ.cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris),PROGRESS IN NATURAL SCIENCE,2009,19(7):837-844
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Mai, WJ]'s Articles
[Hu, CQ]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Mai, WJ]‘s Articles
[Hu, CQ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院南海海洋研究所 - Feedback
Powered by CSpace