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cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)
Mai, WJ; Hu, CQ; chqhu@scsio.ac.cn
2009
Source PublicationPROGRESS IN NATURAL SCIENCE
ISSN1002-0071
Volume19Issue:7Pages:837-844
AbstractThe gene coding for lysozyme in blue shrimp (Litopenaeus stylirostris) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. merguiensi lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 15% SDS-PAGE. Semi-quantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of haemocytes, gill, gonad and the lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after the heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in haemolymph challenged by Micrococcus luteus and V. alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post-stimulation, respectively, and then dropped back to the original level. Bacteriolytic activity of the lysozyme against different bacterial cultures was determined by the solid phase and turbidimetric assays. The results demonstrated that the lysozyme we obtained was not only against Gram-positive and Gram-negative bacteria but also against shrimp pathogens V. alginolyticus and V. parahemolyticus. In addition, the study of the inhibition mechanism revealed that the antibacterial activity of the lysozyme was a result of the bactericidal effect. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
Department[Mai, Weijun; Hu, Chaoqun] Sci Acad China, S China Sea Inst Oceanol, Appl Marine Biol Lab, Guangzhou 510301, Guangdong, Peoples R China; [Mai, Weijun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
KeywordLysozyme c Litopenaeus Stylirostris Pcr-cloning Expression Antibacterial Activity
Subject AreaMaterials Science, Multidisciplinary ; Multidisciplinary Sciences
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Indexed Bysci
Funding ProjectLMB
WOS IDWOS:000266345100007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/5110
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorchqhu@scsio.ac.cn
Recommended Citation
GB/T 7714
Mai, WJ,Hu, CQ,chqhu@scsio.ac.cn. cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)[J]. PROGRESS IN NATURAL SCIENCE,2009,19(7):837-844.
APA Mai, WJ,Hu, CQ,&chqhu@scsio.ac.cn.(2009).cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris).PROGRESS IN NATURAL SCIENCE,19(7),837-844.
MLA Mai, WJ,et al."cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)".PROGRESS IN NATURAL SCIENCE 19.7(2009):837-844.
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