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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge
作者: Wang, ZL ; Wu, ZH ; Jian, JC ; Lu, YS
通讯作者: wuzh@gdou.edu.cn
关键词: SSH ; EST ; RACE ; Bacterial challenge ; Real-time PCR
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2009
卷: 26, 期:4, 页:639-645
收录类别: sci
部门归属: [Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China; [Wang, Zhongliang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Wang, Zhongliang] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; [Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
项目归属: LMB
摘要: The cDNA of pearl oyster Pinctada fucata Hsp70 (designated PFHsp70) was cloned by EST and rapid amplification of cDNA ends (RACE) techniques. The full length of PFHsp70 cDNA was 2376 bp, consisting of a 5'-terminal untranslated region (UTR) of 89 bp, a 3' terminal UTR of 328 bp, and an open reading frame (ORF) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kDa and an estimated isoelectric point of 5.18. BLAST analysis revealed that the PFHsp70 gene shared high similarity with other Hsp70 genes. PFHsp70 contained all the three classical Hsp70 family signatures. The results indicated that the PFHsp70 was a member of the heat shock protein 70 family. Fluorescent real-time quantitative RT-PCR was used to examine the expression of PFHsp70 gene in haemocytes of P. fucata after the challenge of bacteria Vibrio alginolyticus. There was a clear time-dependent expression pattern of PFHsp70 after bacterial challenge, and the mRNA expression reached a maximum level at 4 h post-challenge, which returned to control level after 32 h. The up-regulated mRNA expression of PFHsp70 in P. fucata after bacterial challenge indicates that the Hsp70 gene is inducible and involved in immune response. (C) 2008 Published by Elsevier Ltd.
原文出处: 查看原文
WOS记录号: WOS:000266194500010
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5114
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wang, ZL; Wu, ZH; Jian, JC; Lu, YS.Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge,FISH & SHELLFISH IMMUNOLOGY,2009,26(4):639-645
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文件名: Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge .pdf
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