Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge
Wang, ZL; Wu, ZH; Jian, JC; Lu, YS; wuzh@gdou.edu.cn
2009
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号26期号:4页码:639-645
摘要The cDNA of pearl oyster Pinctada fucata Hsp70 (designated PFHsp70) was cloned by EST and rapid amplification of cDNA ends (RACE) techniques. The full length of PFHsp70 cDNA was 2376 bp, consisting of a 5'-terminal untranslated region (UTR) of 89 bp, a 3' terminal UTR of 328 bp, and an open reading frame (ORF) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kDa and an estimated isoelectric point of 5.18. BLAST analysis revealed that the PFHsp70 gene shared high similarity with other Hsp70 genes. PFHsp70 contained all the three classical Hsp70 family signatures. The results indicated that the PFHsp70 was a member of the heat shock protein 70 family. Fluorescent real-time quantitative RT-PCR was used to examine the expression of PFHsp70 gene in haemocytes of P. fucata after the challenge of bacteria Vibrio alginolyticus. There was a clear time-dependent expression pattern of PFHsp70 after bacterial challenge, and the mRNA expression reached a maximum level at 4 h post-challenge, which returned to control level after 32 h. The up-regulated mRNA expression of PFHsp70 in P. fucata after bacterial challenge indicates that the Hsp70 gene is inducible and involved in immune response. (C) 2008 Published by Elsevier Ltd.
部门归属[Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China; [Wang, Zhongliang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Wang, Zhongliang] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; [Wang, Zhongliang; Wu, Zaohe; Jian, Jichang; Lu, Yishan] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
关键词Ssh Est Race Bacterial Challenge Real-time Pcr
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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收录类别sci
资助项目LMB
WOS记录号WOS:000266194500010
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被引频次:60[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5114
专题中科院海洋生物资源可持续利用重点实验室
通讯作者wuzh@gdou.edu.cn
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Wang, ZL,Wu, ZH,Jian, JC,et al. Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(4):639-645.
APA Wang, ZL,Wu, ZH,Jian, JC,Lu, YS,&wuzh@gdou.edu.cn.(2009).Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge.FISH & SHELLFISH IMMUNOLOGY,26(4),639-645.
MLA Wang, ZL,et al."Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge".FISH & SHELLFISH IMMUNOLOGY 26.4(2009):639-645.
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