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学科主题: Biotechnology & Applied Microbiology; Marine & Freshwater Biology
题名: Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus
作者: Chen, C ; Xie, J ; Hu, CQ
通讯作者: chen.chang@scsio.ac.cn
关键词: colony morphology ; phase variation ; biofilm ; polysaccharide ; Vibrio alginolyticus ; motility
刊名: BIOFOULING
发表日期: 2009
卷: 25, 期:6, 页:525-531
收录类别: sci
部门归属: [Chen Chang; Xie Jin; Hu Chaoqun] Chinese Acad Sci, S China Sea Inst Oceanol, LMB & LAMB, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Many pathogens undergo phase variation between rugose and smooth colony morphology or between opaque and translucent colony morphology, which is mainly due to the variation in the surface polysaccharides. In this study, Vibrio alginolyticus ZJ-51 displayed phase variation between opaque, rugose colonies (Op) and translucent, smooth colonies (Tr). Unlike the vibrios reported previously, Tr cells of ZJ-51 enhanced biofilm formation and motility, but they did not differ from Op cells in the quantity of surface polysaccharides produced. Real time PCR was used to analyze the expression of the genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system. The results revealed that the K-antigen capsule gene cluster (which consists of homologs to the cpsA-K in Vibrio parahaemolyticus) and O-antigen polysaccharide gene cluster (which contains homologs to the wza-wzb-wzc) were significantly more transcribed in Tr cells. The AI-2 quorum-sensing genes showed enhanced expression in the Tr variant which also exhibited greater expression of genes associated with polar flagellar biosynthesis. These results suggest that colony phase variation might affect the virulence and survival ability in the stressful environment inhabited by V. alginolyticus.
原文出处: 查看原文
WOS记录号: WOS:000265633300005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5120
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Chen, C; Xie, J; Hu, CQ.Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus,BIOFOULING,2009,25(6):525-531
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