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学科主题: Marine & Freshwater Biology; Zoology
题名: Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis
作者: Xia, JJ ; Yu, ZI ; Kong, XY
通讯作者: carlzyu@scsio.ac.cn
刊名: JOURNAL OF MOLLUSCAN STUDIES
发表日期: 2009
卷: 75, 页:139-146
收录类别: sci
部门归属: [Xia, Jianjun; Yu, Ziniu; Kong, Xiaoyu] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Appl Marine Biol, Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: We have developed a PCR-RFLP assay of two mitochondrial [16S rDNA and cytochrome oxidase I (COI)] and one nuclear (ITS-1) gene fragment for the identification of seven Crassostrea oysters from the South China Sea. These include the morphologically distinct Crassostrea sikamea and three pairs of morphologically similar species: Crassostrea hongkongensis and Crassostrea ariakensis, Crassostrea gigas and Crassostrea angulata, Crassostrea iredalei and an unidentified Crassostrea sp. All seven species can be distinguished with both mitochondrial markers: DdeI/DraI or AluI/MseI digestion of 16S rDNA and NisI/AluI or NisI/DdeI digestion of COI. Five of the seven species can be distinguished using a single endonuclease digestion of the ITS-1 fragment. Based on genetic distance estimates and on morphology, the newly discovered Crassostrea sp. appears most closely related to C. iredalei. Despite the existence of intra-specific variation at low frequency in some restriction sites, the PCR-RFLP assay developed here can be reliably applied to identify all seven oyster species.
原文出处: 查看原文
WOS记录号: WOS:000265274300007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5122
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Xia, JJ; Yu, ZI; Kong, XY.Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis,JOURNAL OF MOLLUSCAN STUDIES,2009,75():139-146
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