Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis
Xia, JJ; Yu, ZI; Kong, XY; carlzyu@scsio.ac.cn
2009
发表期刊JOURNAL OF MOLLUSCAN STUDIES
ISSN0260-1230
卷号75页码:139-146
摘要We have developed a PCR-RFLP assay of two mitochondrial [16S rDNA and cytochrome oxidase I (COI)] and one nuclear (ITS-1) gene fragment for the identification of seven Crassostrea oysters from the South China Sea. These include the morphologically distinct Crassostrea sikamea and three pairs of morphologically similar species: Crassostrea hongkongensis and Crassostrea ariakensis, Crassostrea gigas and Crassostrea angulata, Crassostrea iredalei and an unidentified Crassostrea sp. All seven species can be distinguished with both mitochondrial markers: DdeI/DraI or AluI/MseI digestion of 16S rDNA and NisI/AluI or NisI/DdeI digestion of COI. Five of the seven species can be distinguished using a single endonuclease digestion of the ITS-1 fragment. Based on genetic distance estimates and on morphology, the newly discovered Crassostrea sp. appears most closely related to C. iredalei. Despite the existence of intra-specific variation at low frequency in some restriction sites, the PCR-RFLP assay developed here can be reliably applied to identify all seven oyster species.
部门归属[Xia, Jianjun; Yu, Ziniu; Kong, Xiaoyu] Chinese Acad Sci, S China Sea Inst Oceanol, Lab Appl Marine Biol, Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
学科领域Marine & Freshwater Biology ; Zoology
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收录类别sci
资助项目LMB
WOS记录号WOS:000265274300007
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5122
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Xia, JJ,Yu, ZI,Kong, XY,et al. Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis[J]. JOURNAL OF MOLLUSCAN STUDIES,2009,75:139-146.
APA Xia, JJ,Yu, ZI,Kong, XY,&carlzyu@scsio.ac.cn.(2009).Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis.JOURNAL OF MOLLUSCAN STUDIES,75,139-146.
MLA Xia, JJ,et al."Identification of seven Crassostrea oysters from the South China Sea using PCR-RFLP analysis".JOURNAL OF MOLLUSCAN STUDIES 75(2009):139-146.
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