Intraspecific genetic variation in mitochondrial 16S rRNA and COI genes in domestic and wild populations of Huaguizhikong scallop Chlamys nobilis Reeve
Yuan, T; He, MX; Huang, LM; hmx@scsio.ac.cn; hlm@scsio.ac.cn
2009
发表期刊AQUACULTURE
ISSN0044-8486
卷号289期号:40545页码:19-25
摘要A 468 base-pair fragment of the mitochondrial 16S rRNA gene and a 481 base-pair fragment of the mitochondrial COI gene were used to study the geographic structure and genetic diversity within Huaguizhikong scallop Chlamys nobilis Reeve. The samples were collected from one Thailand population (TH) which sequences were obtained from the GenBank database, five domestic stocks [Fujian (FJ), Guangxi (GX), Hainan (HN), Daya Bay (DW), and Zhanjiang (ZJ)] and two wild populations [Guangxi (GY) and Daya Bay (DY)]. Sixty polymorphic sites were found in COI gene, and thirty-nine haplotypes were detected in all samples. In addition, twenty-four polymorphic sites were found, and twenty-seven haplotypes were detected in all samples of 16S rRNA gene. The analysis of haplotype frequency distribution and molecular variation indicated that genetic variation and geographic structure were significant. To COI gene and combined data set, the majority (COI: 78.7%, combined data set: 78.5%) of the genetic variation was distributed within populations, furthermore, less than 37.0% genetic variation of 16S rRNA could be distributed within populations. Variance of 16S rRNA gene (4.3%), COI gene (26.1%), and combined data set (23.4%) could be attributed among different geographic regions. The richest variation of 16S rRNA was observed in TH population among the eight populations supported the fact that TH has richer genetic polymorphism than Chinese population, which may sustain the idea of stock introduction. Otherwise, in the present study, we found that the gene diversity (Hd) level of wild population was higher than cultured populations of C. nobilis in three data sets. Our results indicated that genetic composition of cultured C. nobilis populations in southern China have been significantly affected by hatchery performance. Besides, the FJ stock which was the center seed stock of scallop C. nobilis cultured populations has been preliminarily confirmed in our research. (C) 2009 Elsevier B.V. All rights reserved.
部门归属[Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, LMB, LAMB, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Tao] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
关键词Chlamys Nobilis Reeve Genetic Variation Geographic Structure 16s Rrna Gene Coi Gene
学科领域Fisheries ; Marine & Freshwater Biology
URL查看原文
收录类别sci
资助项目LMB
WOS记录号WOS:000264942400004
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5132
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn; hlm@scsio.ac.cn
推荐引用方式
GB/T 7714
Yuan, T,He, MX,Huang, LM,et al. Intraspecific genetic variation in mitochondrial 16S rRNA and COI genes in domestic and wild populations of Huaguizhikong scallop Chlamys nobilis Reeve[J]. AQUACULTURE,2009,289(40545):19-25.
APA Yuan, T,He, MX,Huang, LM,hmx@scsio.ac.cn,&hlm@scsio.ac.cn.(2009).Intraspecific genetic variation in mitochondrial 16S rRNA and COI genes in domestic and wild populations of Huaguizhikong scallop Chlamys nobilis Reeve.AQUACULTURE,289(40545),19-25.
MLA Yuan, T,et al."Intraspecific genetic variation in mitochondrial 16S rRNA and COI genes in domestic and wild populations of Huaguizhikong scallop Chlamys nobilis Reeve".AQUACULTURE 289.40545(2009):19-25.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Intraspecific geneti(386KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。