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学科主题: Virology
题名: Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8
作者: Li, X ; Zhou, R ; Wang, YS ; Sheng, HY ; Tian, XG ; Li, HT ; Qiu, HL
通讯作者: xiao_2005@hotmail.com ; zhou3218@yahoo.com ; yswang@scsio.ac.cn ; shy-gir4020@163.com ; tianxingui7902@yahoo.com.cn ; lht05l02000@yahoo.com.cn ; qtyunying@yahoo.com
关键词: Norovirus ; Capsid protein ; Monoclonal antibodies ; Epitope
刊名: VIRUS RESEARCH
发表日期: 2009
卷: 140, 期:40545, 页:188-193
收录类别: sci
部门归属: [Zhou, Rong; Sheng, Huiying; Tian, Xingui; Li, Haitao; Qiu, Hongling] S China Inst Med Virol, Guangzhou 510663, Guangdong, Peoples R China; [Li, Xiao] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Li, Xiao; Wang, Youshao] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Norovirus is an important cause of acute non-bacterial gastroenteritis in humans. The norovirus genus is comprised of at least five genogroups based on sequence differences. The norovirus genogroup II (GII/4) strain is recognized as the predominant genotype worldwide. We expressed a 60 kDa full-length recombinant capsid protein of norovirus GII/4 in Escherichia coli and generated three monoclonal antibodies (MAbs) against it. Western blotting indicated that all three MAbs had reactivity against the recombinant capsid protein and a 58 kDa native capsid protein of norovirus obtained from stool samples. MAb-capture ELISA showed that MAb detected segmental strains within GII antigens in clinical material. To identify the existent range of this epitope, epitope analyses were processed by expressing 12 amino acids of the GST-fusion peptides. The epitope analyses revealed that the MAb N2C3 recognized a continuous native epitope (WIRNNF60)-W-55 in the shell domain, which not only belongs to strain GII/4, but also to strains GII/7 and GII/8. This is a new native epitope to be reported for norovirus GII/4. (C) 2008 Elsevier B.V. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000264650600025
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5136
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Li, X; Zhou, R; Wang, YS; Sheng, HY; Tian, XG; Li, HT; Qiu, HL.Identification and characterization of a native epitope common to norovirus strains GII/4, GII/7 and GII/8,VIRUS RESEARCH,2009,140(40545):188-193
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文件名: Identification and characterization of a native epitope common to norovirus strains GII-4, GII-7 and GII-8 .pdf
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