Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC
Bai, CC; Han, SY; Chai, XY; Jiang, Y; Li, P; Tu, PF; pengfeitu@vip.163.com
2009
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
卷号32期号:2页码:242-260
摘要With continuous development in analytical instruments over recent years, high performance liquid chromatography ( HPLC) with various detectors has become very important tools for routine analysis and quality control of TCMs and botanical medicines. As a new "mass'' detector, charged aerosol detector ( CAD) provides an alternative detection method, which ultraviolet (UV) is unable to achieve for components owning almost no chromophore in its chemical structures with the lower sensitivity. With the purpose of evaluating the applicability of CAD for the analysis of TCMs, an HPLC-CAD method for simultaneous determination of saponins in Radix et Rhizoma Notoginseng ("Sanqi'' in chinese) was established in this contribution, which was successfully applied for quantitation of seven saponins, notoginsenoside R-1, ginsenosides Rg(1), Re, Rb-1, Rg(2), Rh-1, and Rd, in thirty batches of Sanqi samples. Meanwhile, the LODs and LOQs of UV, ELSD and CAD were compared and the results showed that the CAD method exhibited a lower LOD ( 0.01 similar to 0.15 mu g) and LOQ ( 0.04 similar to 0.41 mu g) than UV and ELSD. Furthermore, the CAD exhibited a steadier baseline in gradient elution compared with UV at 203 nm. Also, the HPLC-CAD method presents many apparent advantages of high sensitivity, steady baseline in gradient elution, easy operating, and it was expected to be a sensitive and universal method for analysis of TCMs containing weak UV absorption compounds. It was demonstrated that the content of seven saponins in Radix et Rhizoma Notoginseng did not significantly correlate with the original work nor with the conventional quality standard.
部门归属[Tu, Peng-Fei] Peking Univ, Hlth Sci Ctr, Modern Res Ctr TCM, Sch Pharmaceut Sci,State Key Lab Nat & Biomimet D, Beijing 100083, Peoples R China; [Bai, Chang-Cai; Li, Ping; Tu, Peng-Fei] China Pharmaceut Univ, Minist Educ, Key Lab Modern Chinese Med, Nanjing 210009, Peoples R China; [Bai, Chang-Cai; Li, Ping; Tu, Peng-Fei] China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210009, Peoples R China; [Chai, Xing-Yun] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China
关键词Charged Aerosol Detector Evaporative Light Scattering Detector Hplc Panax Notoginseng Radix Et Rhizoma Notoginseng Saponins
学科领域Biochemical Research Methods ; Chemistry, Analytical
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收录类别sci
资助项目LMB
WOS记录号WOS:000261875000006
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5156
专题中科院海洋生物资源可持续利用重点实验室
通讯作者pengfeitu@vip.163.com
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GB/T 7714
Bai, CC,Han, SY,Chai, XY,et al. Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(2):242-260.
APA Bai, CC.,Han, SY.,Chai, XY.,Jiang, Y.,Li, P.,...&pengfeitu@vip.163.com.(2009).Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(2),242-260.
MLA Bai, CC,et al."Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.2(2009):242-260.
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