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Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography
Long, LJ; Song, Y; Wu, J; Lei, L; Huang, K; Long, BW; wwujun2003@yahoo.com
2006
发表期刊ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
卷号386期号:40732页码:2169-2174
摘要In our research into chlorophylls of marine dinoflagellates, chlorophyll a was separated rapidly from the hexane extract of Amphidinium carterae in three steps. The first step was silica gel column chromatography, where elution was performed with 0-50% ethyl acetate in n-hexane. The second was high-speed counter-current chromatography using a two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (5:5:5:1, v/v), and the third step was preparative reversed-phase high-performance liquid chromatography using a solvent system of acetone-water (89:11, v/v). HPLC analysis showed that the purity of chlorophyll a from the second step was over 83%, and after the third it was over 99%. Thirty milligrams of chlorophyll a was isolated from a crude sample of 250 mg of chlorophylls, and its structure was identified by analyzing its MS, H-1 NMR and C-13 NMR spectra.
部门归属Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Medica, Guangzhou 510301, Peoples R China; Sinphar Tian Li Pharmaceut Co Ltd, Yuhang Econ Dev Area, Hangzhou 311100, Peoples R China
关键词Amphidinium Carterae High-speed Counter-current Chromatography Reversed-phase High-performance Liquid Chromatography Preparative Chromatography Chlorophyll a
学科领域Biochemical Research Methods ; Chemistry, Analytical
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收录类别sci
资助项目广东省海洋药物重点实验室
WOS记录号WOS:000242144200027
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5168
专题广东省海洋药物重点实验室
通讯作者wwujun2003@yahoo.com
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Long, LJ,Song, Y,Wu, J,et al. Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(40732):2169-2174.
APA Long, LJ.,Song, Y.,Wu, J.,Lei, L.,Huang, K.,...&wwujun2003@yahoo.com.(2006).Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,386(40732),2169-2174.
MLA Long, LJ,et al."Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386.40732(2006):2169-2174.
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