中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 广东省海洋药物重点实验室  > 期刊论文
学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography
作者: Long, LJ ; Song, Y ; Wu, J ; Lei, L ; Huang, K ; Long, BW
通讯作者: wwujun2003@yahoo.com
关键词: Amphidinium carterae ; high-speed counter-current chromatography ; reversed-phase high-performance liquid chromatography ; preparative chromatography ; chlorophyll a
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2006
卷: 386, 期:40732, 页:2169-2174
收录类别: sci
部门归属: Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Medica, Guangzhou 510301, Peoples R China; Sinphar Tian Li Pharmaceut Co Ltd, Yuhang Econ Dev Area, Hangzhou 311100, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: In our research into chlorophylls of marine dinoflagellates, chlorophyll a was separated rapidly from the hexane extract of Amphidinium carterae in three steps. The first step was silica gel column chromatography, where elution was performed with 0-50% ethyl acetate in n-hexane. The second was high-speed counter-current chromatography using a two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (5:5:5:1, v/v), and the third step was preparative reversed-phase high-performance liquid chromatography using a solvent system of acetone-water (89:11, v/v). HPLC analysis showed that the purity of chlorophyll a from the second step was over 83%, and after the third it was over 99%. Thirty milligrams of chlorophyll a was isolated from a crude sample of 250 mg of chlorophylls, and its structure was identified by analyzing its MS, H-1 NMR and C-13 NMR spectra.
原文出处: 查看原文
WOS记录号: WOS:000242144200027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5168
Appears in Collections:广东省海洋药物重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current .pdf(149KB)----开放获取--View Download

Recommended Citation:
Long, LJ; Song, Y; Wu, J; Lei, L; Huang, K; Long, BW.Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(40732):2169-2174
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Long, LJ]'s Articles
[Song, Y]'s Articles
[Wu, J]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Long, LJ]‘s Articles
[Song, Y]‘s Articles
[Wu, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current .pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace