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Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line
Wang, F; Ma, RD; Yu, LJ; ywyj9578@sohu.com
2006
发表期刊CANCER CHEMOTHERAPY AND PHARMACOLOGY
ISSN0344-5704
卷号57期号:3页码:389-399
摘要Background: Tubeimoside I (TBMS1), a triterpenoid saponin, isolated from the tubers of Bolbostemma paniculatum, showed potent antitumor and antitumor-promoting effects. The objective of this study is to investigate the role of mitochondria and mitochondria cytochrome c in TBMS1-mediated apoptosis of human cervical carcinoma HeLa cell line. Methods: Viability of HeLa cells was measured by MTT assay. Apoptotic induction by TBMS1 was determined by fluorescence microscopy, flow cytometry and gel electrophoresis of fragmented DNA. Mitochondrial transmembrane potential (triangle psi m) was assayed by flow cytometry. Cytochrome c (Cyt c) was detected by Western blotting. Results: The results showed that Cyclosporin A (CsA) partly protected HeLa cells from growth inhibitory effect of TBMS1, and partly countered the ability of TBMS1 to rapidly induce apoptosis in HeLa cells, and that TBMS1 decreased triangle psi m and induced Cyt c release by a mechanism inhibited by CsA, and that TBMS1 induced apoptosis of HeLa cells dose-dependently in accordance with increase of cytosolic Cyt c. Conclusions: TBMS1 opens the permeability transition (PT) pore, thereby decreasing triangle psi m, releasing Cyt c from mitochondria, and further causing a series of events consistent with established mechanistic models of apoptosis.
部门归属Zhanjiang Ocean Univ, Guangdong Provincial Key Lab Marine Mat Med, Zhanjiang 524025, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Grad Sch, Guangzhou, Peoples R China
关键词Tubeimoside i Mitochondria Cytochrome c Apoptosis Hela Cells
学科领域Oncology ; Pharmacology & Pharmacy
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收录类别sci
资助项目广东省海洋药物重点实验室
WOS记录号WOS:000234201200015
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被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5208
专题广东省海洋药物重点实验室
通讯作者ywyj9578@sohu.com
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Wang, F,Ma, RD,Yu, LJ,et al. Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line[J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY,2006,57(3):389-399.
APA Wang, F,Ma, RD,Yu, LJ,&ywyj9578@sohu.com.(2006).Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line.CANCER CHEMOTHERAPY AND PHARMACOLOGY,57(3),389-399.
MLA Wang, F,et al."Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line".CANCER CHEMOTHERAPY AND PHARMACOLOGY 57.3(2006):389-399.
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