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Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.
Jiang, SM; Sun, W; Chen, MJ; Dai, SK; Zhang, L; Liu, YH; Lee, KJ; Li, X; lixiang@scsio.ac.cn
2007
发表期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
卷号92期号:4页码:405-416
摘要This study describes actinobacteria isolated from the marine sponge Haliclona sp. collected in shallow water of the South China Sea. A total of 54 actinobacteria were isolated using media selective for actinobacteria. Species diversity and natural product diversity of isolates from marine sponge Haliclona sp. were analysed. Twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis. The 16S rRNA genes of 24 isolates were digested by restriction enzymes TaqI and MspI and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Streptomyces, Nocardiopsis, Micromonospora and Verrucosispora; one other isolate was recovered that does not belong to known genera based on its unique 16S rRNA gene sequence. To our knowledge, this is the first report of a bacterium classified as Verrucosispora sp. that has been isolated from a marine sponge. The majority of the strains tested belong to the genus Streptomyces and three isolates may be new species. All of the 24 isolates were screened for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). PKS and NRPS sequences were detected in more than half of the isolates and the different "PKS-I-PKS-II-NRPS" combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution.
部门归属Chinese Acad Sci, So China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Marine Bioresources Sust Utiliz, Guangzhou 510301, Peoples R China; Guangzhou Acad Agr Sci, Floricult Res Inst, Guangzhou, Peoples R China; Hannam Univ, Dept Biol, Taejon, South Korea
关键词Marine Sponge Actinobacteria 16s Rrna Rflp Pks Nrps
学科领域Microbiology
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收录类别sci
资助项目广东省海洋药物重点实验室
WOS记录号WOS:000249729500004
引用统计
被引频次:69[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5220
专题广东省海洋药物重点实验室
通讯作者lixiang@scsio.ac.cn
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GB/T 7714
Jiang, SM,Sun, W,Chen, MJ,et al. Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2007,92(4):405-416.
APA Jiang, SM.,Sun, W.,Chen, MJ.,Dai, SK.,Zhang, L.,...&lixiang@scsio.ac.cn.(2007).Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp..ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,92(4),405-416.
MLA Jiang, SM,et al."Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 92.4(2007):405-416.
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