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学科主题: Microbiology
题名: Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.
作者: Jiang, SM ; Sun, W ; Chen, MJ ; Dai, SK ; Zhang, L ; Liu, YH ; Lee, KJ ; Li, X
通讯作者: lixiang@scsio.ac.cn
关键词: marine sponge ; actinobacteria ; 16S rRNA ; RFLP ; PKS ; NRPS
刊名: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
发表日期: 2007
卷: 92, 期:4, 页:405-416
收录类别: sci
部门归属: Chinese Acad Sci, So China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Marine Bioresources Sust Utiliz, Guangzhou 510301, Peoples R China; Guangzhou Acad Agr Sci, Floricult Res Inst, Guangzhou, Peoples R China; Hannam Univ, Dept Biol, Taejon, South Korea
项目归属: 广东省海洋药物重点实验室
摘要: This study describes actinobacteria isolated from the marine sponge Haliclona sp. collected in shallow water of the South China Sea. A total of 54 actinobacteria were isolated using media selective for actinobacteria. Species diversity and natural product diversity of isolates from marine sponge Haliclona sp. were analysed. Twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis. The 16S rRNA genes of 24 isolates were digested by restriction enzymes TaqI and MspI and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Streptomyces, Nocardiopsis, Micromonospora and Verrucosispora; one other isolate was recovered that does not belong to known genera based on its unique 16S rRNA gene sequence. To our knowledge, this is the first report of a bacterium classified as Verrucosispora sp. that has been isolated from a marine sponge. The majority of the strains tested belong to the genus Streptomyces and three isolates may be new species. All of the 24 isolates were screened for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). PKS and NRPS sequences were detected in more than half of the isolates and the different "PKS-I-PKS-II-NRPS" combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution.
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WOS记录号: WOS:000249729500004
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5220
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Recommended Citation:
Jiang, SM; Sun, W; Chen, MJ; Dai, SK; Zhang, L; Liu, YH; Lee, KJ; Li, X.Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.,ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2007,92(4):405-416
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