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Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp nov.
Dastager, SG; Dayanand, A; Li, WJ; Kim, CJ; Lee, JC; Park, DJ; Tian, XP; Raziuddin, QS
2008
发表期刊CURRENT MICROBIOLOGY
ISSN0343-8651
卷号57期号:6页码:638-642
摘要Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45C. The enzyme preparation exhibited activity over a broad range of pH (4-12) and temperature (27-55C). Optimum activity was observed at a pH of 9.0 and a temperature of 45C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme activity. Two active zones in the range of 19 to 35 kDa were detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
部门归属[Dastager, S. G.; Dayanand, Agasar; Raziuddin, Q. S.] Gulbarga Univ, Dept Studies & Res Microbiol, Gulbarga 585106, Karnataka, India; [Dastager, S. G.; Li, Wen-Jun; Tian, Xin-Peng] Yunnan Univ, Yunnan Inst Microbiol, Lab Conservat & Utilizat Bio Resources, Kunming 650091, Renfrew, Peoples R China; [Tian, Xin-Peng] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Medica, Guangzhou 510301, Guangdong, Peoples R China; [Dastager, S. G.; Kim, Chang-Jin; Lee, Jae-Chan; Park, Dong-Jin] Korea Res Inst Biosci & Biotechnol, Taejon 305333, South Korea
学科领域Microbiology
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收录类别sci
资助项目广东省海洋药物重点实验室
WOS记录号WOS:000261177900022
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5248
专题广东省海洋药物重点实验室
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Dastager, SG,Dayanand, A,Li, WJ,et al. Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp nov.[J]. CURRENT MICROBIOLOGY,2008,57(6):638-642.
APA Dastager, SG.,Dayanand, A.,Li, WJ.,Kim, CJ.,Lee, JC.,...&Raziuddin, QS.(2008).Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp nov..CURRENT MICROBIOLOGY,57(6),638-642.
MLA Dastager, SG,et al."Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp nov.".CURRENT MICROBIOLOGY 57.6(2008):638-642.
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