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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography
作者: Yin, H ; Zhang, S ; Luo, XM ; Liu, YH
通讯作者: zhs_1999@hotmail.com
关键词: benzoxazinoids ; (2R)-2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one (HBOA-Glc) ; (2R)-2-O-beta-D-glucopyranosyl-4-hydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA-Glc) ; high-speed counter-current chromatography (HSCCC) ; Acanthus ilicifolius L
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2008
卷: 1205, 期:40545, 页:177-181
收录类别: sci
部门归属: [Yin, Hao; Zhang, Si; Luo, Xiongming; Liu, Yonghong] Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Yin, Hao; Zhang, Si] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
项目归属: 广东省海洋药物重点实验室
摘要: The first preparative separation of two benzoxazinoids, (2R)-2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one (HBOA-Glc) and (2R)-2-O-beta-D-glucopyranosyl-4-hydroxy-2H-1,4-benzoxazin3(4H)-one (DIBOA-Glc), by means of high-speed counter-current chromatography (HSCCC) from the n-butanol extract of Acanthus ilicifolius L. is presented. The two-phase solvent system containing ethyl acetate-n-butanol-0.5%NH4OH (2:3:5, v/v/v, system 13) was selected for the one-step HSCCC separation of HBOA-Glc and DIBOA-Glc according to the partition coefficient values (K) for target compounds and the separation factor (alpha) between the two target compounds. In the one-step HSCCC separation using solvent B, from 100 mg n-butanol extract of A. ilicifolius, 6.3 mg HBOA-Glc and 6.8 mg DIBOA-Glc were isolated with purities of 90.3% and 80.2%, respectively. In order to obtain the two target compounds with higher purity, a second separation process was developed comprising two steps. In the two-step separation, the sample was first pre-purified by HSCCC using ethyl acetate-n-butanol-water (2:3:5, v/v/v, system A) solvent system and then purified using solvent system B. A 100-mg amount of the n-butanol extracts of A. ilicifolius was separated to yield 5.8 mg of HBOA-Glc and 4.8 mg of DIBOA-Glc with purities of 97.1% and 94.8%, respectively, which were directly used for NMR analyses. (C) 2008 Elsevier B.V. All rights reserved.
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WOS记录号: WOS:000259884700022
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5262
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Recommended Citation:
Yin, H; Zhang, S; Luo, XM; Liu, YH.Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography,JOURNAL OF CHROMATOGRAPHY A,2008,1205(40545):177-181
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