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学科主题: Biochemistry & Molecular Biology
题名: Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis
作者: Zhang, CS ; Bitto, E ; Goff, RD ; Singh, S ; Bingman, CA ; Griffith, BR ; Albermann, C ; Phillips, GN ; Thorson, JS
通讯作者: phillips@biochem.wisc.edu ; jsthorson@pharmacy.wisc.edu
刊名: CHEMISTRY & BIOLOGY
发表日期: 2008
卷: 15, 期:8, 页:842-853
收录类别: sci
部门归属: [Bitto, Eduard; Bingman, Craig A.; Phillips, George N., Jr.] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA; [Zhang, Changsheng] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China; [Goff, Randal D.; Singh, Shanteri; Griffith, Byron R.; Albermann, Christoph; Thorson, Jon S.] Univ Wisconsin, Lab Biosynthet Chem, Div Pharmaceut Sci, Sch Pharm,Natl Cooperat Drug Discovery Grp Progra, Madison, WI 53706 USA
项目归属: 广东省海洋药物重点实验室
摘要: The enediyne antibiotic calicheamicin (CLM) gamma(I)(1) is a prominent antitumor agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an aromatic unit and four unusual carbohydrates. Herein we report the heterologous expression and the biochemical characterization of the two "internal" glycosyltransferases CaIG3 and CaIG2 and the structural elucidation of an enediyne glycosyltransferase (CaIG3). In conjunction with the previous characterization of the "external" CLM GTs CaIG1 and CaIG4, this study completes the functional assignment of all four CLM GTs, extends the utility of enediyne GT-catalyzed reaction reversibility, and presents conclusive evidence of a sequential glycosylation pathway in CLM biosynthesis. This work also reveals the common GT-B structural fold can now be extended to include enediyne GTs.
原文出处: 查看原文
WOS记录号: WOS:000258940000012
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5266
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Zhang, CS; Bitto, E; Goff, RD; Singh, S; Bingman, CA; Griffith, BR; Albermann, C; Phillips, GN; Thorson, JS.Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis,CHEMISTRY & BIOLOGY,2008,15(8):842-853
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文件名: Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis .pdf
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