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The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis
Wang, Y; Chen, T; ywang@yic.ac.cn
2008
发表期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
卷号24期号:12页码:2927-2932
摘要The carotenoid composition of the astaxanthin-producing green alga Chlorella zofingiensis was investigated using high-performance liquid chromatography. Astaxanthin, adonixanthin, and zeaxanthin are the major carotenoids in this alga. The pigment pattern was characterized during the accumulation period, and in response to diphenylamine (DPA), an inhibitor of carotenoid biosynthesis. An increase in zeaxanthin followed by a decrease in xanthophyll was seen after the induction of astaxanthin biosynthesis by glucose. This biphasic kinetics of zeaxanthin was parallel to the marked increase in adonixanthin (from 0 mg g(-1) to 0.21 mg g(-1)) and astaxanthin (from 0.05 mg g(-1) to 0.35 mg g(-1)) and decrease of beta-carotene (from 0.30 mg g(-1) to 0.03 mg g(-1)). More importantly, unlike the Haematococcus alga, in which there was a high beta-carotene accumulation after DPA treatment, C. zofingiensis showed an accumulation of extra zeaxanthin instead of beta-carotene after treatment of the cells with DPA. All these results observed in vivo studies corroborate the observations in vitro studies at the enzyme level that zeaxanthin is a substrate for the carotenoid oxygenase in C. zofingiensis. It is suggested that zeaxanthin might be an important intermediate and not an end product of the biosynthetic pathway of astaxanthin. Therefore, a new pathway for astaxanthin formation by C. zofingiensis, which is different from that of the other astaxanthin-producing microorganisms, is proposed. An understanding of the astaxanthin biosynthetic pathway may yield important information toward the optimization of astaxanthin production, especially for the improvement of astaxanthin through genetic manipulations.
部门归属[Wang, Yan] Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China; [Wang, Yan; Chen, Tao] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
关键词Astaxanthin Chlorella Zofingiensis Diphenylamine Zeaxanthin Glucose Stress
学科领域Biotechnology & Applied Microbiology
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收录类别sci
资助项目海洋生物
WOS记录号WOS:000260319900023
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5352
专题海洋生物
通讯作者ywang@yic.ac.cn
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Wang, Y,Chen, T,ywang@yic.ac.cn. The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(12):2927-2932.
APA Wang, Y,Chen, T,&ywang@yic.ac.cn.(2008).The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(12),2927-2932.
MLA Wang, Y,et al."The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.12(2008):2927-2932.
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