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学科主题: Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology
题名: Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula
作者: Zhou, YH ; Wu, QP ; Xu, XK ; Yang, XJ ; Ye, YW ; Zhang, JM
通讯作者: mylovelyswallow@yeah.net ; wuqp203@yahoo.com.cn
关键词: Enterobacter sakazakii ; immobilization ; duplex PCR ; powdered infant formula
刊名: FOOD MICROBIOLOGY
发表日期: 2008
卷: 25, 期:5, 页:648-652
收录类别: sci
部门归属: [Zhou, Yanhong; Wu, Qingping; Xu, Xiaoke; Yang, Xiaojuan; Ye, Yingwang; Zhang, Jumei] Guandong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Zhou, Yanhong] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; [Zhou, Yanhong; Ye, Yingwang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Ye, Yingwang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: 海洋生物
摘要: Enterobacter sakazakii is an emerging opportunistic pathogen that is associated with rare but life-threatening cases of meningitis, necrotizing enterocolitis, and sepsis in premature and full-term infants. In the present study, a procedure was developed for immobilization of E. sakazakii with zirconium hydroxide coupled with detection by a species-specific duplex PCR, based on 16s-23s rDNA internal transcribed spacer (ITS) and ompA gene. Specificity of duplex PCR was tested against two-type strains, six isolates of E. sakazakii and other eight non-E. sakazakii species. When pure culture of E. sakazakii was used for immobilization, total recovery rate ranged from 79.4% to 99.6% of input bacteria, and the detection limit of duplex PCR was 3 x 10(5) CFU/ml. Different levels of E. sakazakii were inoculated into 90 ml reconstituted powdered infant formula (PIF), and detection limit of duplex PCR was 3 x 10(0) CFU/ ml with 24-30h enrichment after immobilization. When the experiment was performed in the presence of 10(2) CFU/ml Salmonella typhimurium, the detection limit of duplex PCR was not affected after enrichment. Seven out of 13 commercial PIF were detected positive by duplex PCR after immobilization, while only three were positive by biological methods. This study demonstrates that the combination of immobilization method with duplex PCR is easy, rapid, and efficient, and may have applications for the detection of E. sakazakii in more PIF samples. (C) 2008 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000257628700003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5354
Appears in Collections:海洋生物_期刊论文

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Zhou, YH; Wu, QP; Xu, XK; Yang, XJ; Ye, YW; Zhang, JM.Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula,FOOD MICROBIOLOGY,2008,25(5):648-652
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