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A Comparison of Polymerase Chain Reaction and International Organization for Standardization Methods for Determination of Enterobacter sakazakii Contamination of Infant Formulas from Chinese Mainland Markets
Ye, YW; Wu, QP; Yao, L; Dong, XH; Wu, K; Zhang, JM; wuqp203@yahoo.com.cn
2009
发表期刊FOODBORNE PATHOGENS AND DISEASE
ISSN1535-3141
卷号6期号:10页码:1229-1234
摘要Enterobacter sakazakii is an emerging foodborne pathogen associated with meningitis, necrotizing enterocolitis, and sepsis in infants. One of the main transmission vehicles is the commercially available infant formulas. To provide efficient options and direction for detecting E. sakazakii in infant formulas, evaluation of different polymerase chain reaction (PCR) assays targeting the 16S-23S rDNA internal transcribed spacer (ITS), the ompA gene, and the alpha-1,4-glucosidase gene (gluA) of this organism, were compared to the International Organization for Standardization (ISO) method for detecting E. sakazakii in the 243 commercial infant formula samples. Twelve samples were found to be positive for E. sakazakii by all the PCR assays used, followed by sequencing of PCR products. Ten samples were found to be positive by the ISO method, and all 10 gave positive signals for all the PCR amplifications. In contrast, four false-positive results were generated by single-PCR of the ITS region and one false-positive result targeting the ompA gene, while two false-negative results occurred with the ISO method. Combined with selective enrichment step(s), duplex-PCR targeting ITS and ompA and targeting ompA and gluA genes or single-PCR of the gluA gene can be used to test for contamination by E. sakazakii in infant formulas before they enter the market. PCR techniques will be helpful for routine monitoring and risk assessment for large-scale screenings.
部门归属[Wu, Qingping; Yao, Lin; Dong, Xiaohui; Wu, Kui; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Ye, Yingwang; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; Heifei Univ Technol, Sch Biotechnol & Food Engn, Hefei, Peoples R China; [Ye, Yingwang; Yao, Lin; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China; [Yao, Lin] Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China
学科领域Food Science & Technology
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收录类别sci
资助项目海洋生物
WOS记录号WOS:000272462700009
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5378
专题海洋生物
通讯作者wuqp203@yahoo.com.cn
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Ye, YW,Wu, QP,Yao, L,et al. A Comparison of Polymerase Chain Reaction and International Organization for Standardization Methods for Determination of Enterobacter sakazakii Contamination of Infant Formulas from Chinese Mainland Markets[J]. FOODBORNE PATHOGENS AND DISEASE,2009,6(10):1229-1234.
APA Ye, YW.,Wu, QP.,Yao, L.,Dong, XH.,Wu, K.,...&wuqp203@yahoo.com.cn.(2009).A Comparison of Polymerase Chain Reaction and International Organization for Standardization Methods for Determination of Enterobacter sakazakii Contamination of Infant Formulas from Chinese Mainland Markets.FOODBORNE PATHOGENS AND DISEASE,6(10),1229-1234.
MLA Ye, YW,et al."A Comparison of Polymerase Chain Reaction and International Organization for Standardization Methods for Determination of Enterobacter sakazakii Contamination of Infant Formulas from Chinese Mainland Markets".FOODBORNE PATHOGENS AND DISEASE 6.10(2009):1229-1234.
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