[Ye, Yingwang; Wu, Qingping; Yao, Lin; Dong, Xiaohui; Wu, Kui; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Ye, Yingwang; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ye, Yingwang; Yao, Lin; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Yao, Lin] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
项目归属:
海洋生物
摘要:
Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was employed to generate ERIC-PCR fingerprints of 23 strains of E. sakazakii. A consensus fragment was observed in all 23 strains and was purified, cloned and sequenced from type strain ATCC29544. A comparison of the nucleotide acid with other sequences available in the GenBank revealed 98% of identity with E. sakazakii ATCC BAA-894 and 71%-75% identity with oligopeptidase gene or protease 11 gene of some species from the Enterobacteriaceae family. The consensus fragment from ATCC29544 was used to synthesize probes using DIG-labeling by PCR for dot hybridization. The consensus fragment was found to be highly conserved. Diversity analysis based on the consensus fragment sequencing showed a high heterogeneity between E sakazakii strains and other related strains. In addition, 24 E. sakazakii strains could be distributed into two groups, while E. sakazakii ATCC51329 formed a separate branch. Genetic diversity and potential taxonomic complexity were evident within the E sakazakii strains. (C) 2009 Elsevier B.V. All rights reserved.
Ye, YW; Wu, QP; Yao, L; Dong, XH; Wu, K; Zhang, JM.Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii,INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2009,132(40577):172-175
