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Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii
Ye, YW; Wu, QP; Yao, L; Dong, XH; Wu, K; Zhang, JM; yeyw04@mails.gucas.ac.cn; wuqp203@yahoo.com.cn
2009
发表期刊INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN0168-1605
卷号132期号:40577页码:172-175
摘要Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was employed to generate ERIC-PCR fingerprints of 23 strains of E. sakazakii. A consensus fragment was observed in all 23 strains and was purified, cloned and sequenced from type strain ATCC29544. A comparison of the nucleotide acid with other sequences available in the GenBank revealed 98% of identity with E. sakazakii ATCC BAA-894 and 71%-75% identity with oligopeptidase gene or protease 11 gene of some species from the Enterobacteriaceae family. The consensus fragment from ATCC29544 was used to synthesize probes using DIG-labeling by PCR for dot hybridization. The consensus fragment was found to be highly conserved. Diversity analysis based on the consensus fragment sequencing showed a high heterogeneity between E sakazakii strains and other related strains. In addition, 24 E. sakazakii strains could be distributed into two groups, while E. sakazakii ATCC51329 formed a separate branch. Genetic diversity and potential taxonomic complexity were evident within the E sakazakii strains. (C) 2009 Elsevier B.V. All rights reserved.
部门归属[Ye, Yingwang; Wu, Qingping; Yao, Lin; Dong, Xiaohui; Wu, Kui; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Ye, Yingwang; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ye, Yingwang; Yao, Lin; Dong, Xiaohui; Wu, Kui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Yao, Lin] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
关键词Consensus Fragment Enterobacterial Repetitive Intergenic Consensus (Eric)-pcr Enterobacter Sakazakii Dot Hybridization
学科领域Food Science & Technology ; Microbiology
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收录类别sci
资助项目海洋生物
WOS记录号WOS:000267138700013
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5380
专题海洋生物
通讯作者yeyw04@mails.gucas.ac.cn; wuqp203@yahoo.com.cn
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Ye, YW,Wu, QP,Yao, L,et al. Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2009,132(40577):172-175.
APA Ye, YW.,Wu, QP.,Yao, L.,Dong, XH.,Wu, K.,...&wuqp203@yahoo.com.cn.(2009).Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,132(40577),172-175.
MLA Ye, YW,et al."Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 132.40577(2009):172-175.
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