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学科主题: Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
题名: Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology
作者: Gao, H ; Liu, M ; Liu, JT ; Dai, HQ ; Zhou, XL ; Liu, XY ; Zhuo, Y ; Zhang, WQ ; Zhang, LX
通讯作者: Zhanglixin@im.ac.cn
关键词: Streptomyces avermitilis ; Avermectin B1a ; Medium optimization ; Response surface
刊名: BIORESOURCE TECHNOLOGY
发表日期: 2009
卷: 100, 期:17, 页:4012-4016
收录类别: sci
部门归属: [Gao, Hong; Liu, Mei; Liu, Jintao; Dai, Huanqin; Zhou, Xianlong; Liu, Xiangyang; Zhuo, Ying; Zhang, Wenquan; Zhang, Lixin] Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China; [Zhang, Lixin] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
项目归属: 海洋生物
摘要: Response surface methodology was employed to optimize the composition of medium for the production of avermectin B1a by Streptomyces avermitilis 14-12A in shaker flask cultivation. Corn starch and yeast extract were found to have significant effects on avermectin B1a production by the Plackett-Burman design. The steepest ascent method was used to access the optimal region of the medium composition, followed by an application of response surface. The analysis revealed that the optimum values of the tested variables were 149.57 g/l corn starch and 8.92 g/l yeast extract. A production of 5128 mg/l, which was in agreement with the prediction, was observed in verification experiment. In comparison to the production of original level (3528 mg/l), 1.45-fold increase had been obtained. (C) 2009 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000266752200025
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5390
Appears in Collections:海洋生物_期刊论文

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Recommended Citation:
Gao, H; Liu, M; Liu, JT; Dai, HQ; Zhou, XL; Liu, XY; Zhuo, Y; Zhang, WQ; Zhang, LX.Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology,BIORESOURCE TECHNOLOGY,2009,100(17):4012-4016
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