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学科主题: Biotechnology & Applied Microbiology
题名: Production and characterization of a group of bioemulsifiers from the marine Bacillus velezensis strain H3
作者: Liu, XY ; Ren, BA ; Chen, M ; Wang, HB ; Kokare, CR ; Zhou, XL ; Wang, JD ; Dai, HQ ; Song, FH ; Liu, M ; Wang, JA ; Wang, SJ ; Zhang, LX
通讯作者: zhanglixin@im.ac.cn
关键词: Bacillus velezensis ; Biosurfactant ; Lipopeptide ; Surfactin ; Bioemulsifier
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2010
卷: 87, 期:5, 页:1881-1893
收录类别: sci
部门归属: [Liu, Xiangyang; Ren, Biao; Chen, Ming; Zhou, Xianlong; Dai, Huanqin; Song, Fuhang; Liu, Mei; Zhang, Lixin] Chinese Acad Sci, Inst Microbiol, Beijing 100190, Peoples R China; [Ren, Biao] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Wang, Haibin] Zhejiang Hisun Pharmaceut Co Ltd, Taizhou 318000, Peoples R China; [Kokare, Chandrakant R.] Bharati Vidyapeeth Univ, Poona Coll Pharm, Dept Pharmaceut Biotechnol & Pharmaceut, Pune 411038, Maharashtra, India; [Zhang, Lixin] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Wang, Shujin] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China; [Zhang, Lixin] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510663, Guangdong, Peoples R China; [Chen, Ming] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
项目归属: 海洋生物
摘要: Marine microbes are a rich source of bioactive compounds, such as drugs, enzymes, and biosurfactants. To explore the bioactive compounds from our marine natural product library, an oil emulsification assay was applied to discover biosurfactants and bioemulsifiers. A spore-forming bacterial strain from sea mud was found to produce bioemulsifiers with good biosurfactant activity and a broad spectrum of antimicrobial properties. It was identified as Bacillus velezensis H3 using genomic and phenotypic data analysis. This strain was able to produce biosurfactants with an optimum emulsification activity at pH 6.0 and 2% NaCl by using starch as the carbon source and ammonium sulfate as the nitrogen source. The emulsification-guided isolation and purification procedure led to the discovery of the biosurfactant components, which were mainly composed of nC(14)-surfactin and anteisoC(15)-surfactin as determined by NMR and MS spectra. These compounds can reduce the surface tension of phosphate-buffered saline (PBS) from 71.8 to 24.8 mN/m. The critical micelle concentrations (CMCs) of C-14-surfactin and C-15-surfactin in 0.1 M PBS (pH 8.0) were determined to be 3.06 x 10(-5) and 2.03 x 10(-5) mol/L, respectively. The surface tension values at CMCs for C-14-surfactin and C-15-surfactin were 25.7 and 27.0 mM/m, respectively. In addition, the H3 biosurfactant exhibited antimicrobial activities against Staphyloccocus aureus, Mycobacterium, Klebsiella peneumoniae, Pseudomonas aeruginosa, and Candida albicans. Thus B. velezensis H3 is an alternative surfactin producer with potential application as an industrial strain for the lipopeptide production.
原文出处: 查看原文
WOS记录号: WOS:000280036300030
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5424
Appears in Collections:海洋生物_期刊论文

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Liu, XY; Ren, BA; Chen, M; Wang, HB; Kokare, CR; Zhou, XL; Wang, JD; Dai, HQ; Song, FH; Liu, M; Wang, JA; Wang, SJ; Zhang, LX.Production and characterization of a group of bioemulsifiers from the marine Bacillus velezensis strain H3,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(5):1881-1893
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