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学科主题: Biotechnology & Applied Microbiology; Microbiology
题名: Evaluation of a loop-mediated isothermal amplification method for the rapid detection of Vibrio harveyi in cultured marine shellfish
作者: Cao, YT ; Wu, ZH ; Jian, JC ; Lu, YS
通讯作者: wuzh@gdou.edu.cn
关键词: detection ; loop-mediated isothermal amplification ; toxR gene ; Vibrio harveyi
刊名: LETTERS IN APPLIED MICROBIOLOGY
发表日期: 2010
卷: 51, 期:1, 页:24-29
收录类别: sci
部门归属: [Cao, Y. T.; Wu, Z. H.; Jian, J. C.; Lu, Y. S.] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China; [Cao, Y. T.; Wu, Z. H.; Jian, J. C.; Lu, Y. S.] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Cao, Y. T.; Wu, Z. H.; Jian, J. C.; Lu, Y. S.] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; [Cao, Y. T.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
项目归属: 海洋生物
摘要: Aims: The purpose of this study was to establish a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive detection of Vibrio harveyi in mariculture shellfish. Methods and Results: A set of four primers, two outer and two inner primers, were designed from the toxR gene sequence of V. harveyi. The LAMP reaction was conducted at 65 degrees C for 60 min. There were no cross-reactions with other bacterial strains indicating a high specificity of the LAMP. The detection sensitivity of the LAMP assay for V. harveyi with both of pure cultures and added shellfish cultures is about 10-5 dilution level (equivalent to 17 center dot 2 cells per reaction). The amplification products were detected by visual inspection using SYBR Green I. The detection sensitivity using the LAMP method was 10 times higher than that of conventional PCR. Conclusions: The LAMP assay established in this study is an extremely specific, sensitive and rapid for identification of V. harveyi in mariculture shellfish. Significance and Impact of the Study: This LAMP technique provides an important detecting tool for the detection of V. harveyi infection both in the laboratory and field. This technique is recommended as an applied protocol for health management programme and disease surveillance of in hatcheries as well as in grow-out pond, to prevent the disease outbreak.
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WOS记录号: WOS:000278623300005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5426
Appears in Collections:海洋生物_期刊论文

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Recommended Citation:
Cao, YT; Wu, ZH; Jian, JC; Lu, YS.Evaluation of a loop-mediated isothermal amplification method for the rapid detection of Vibrio harveyi in cultured marine shellfish,LETTERS IN APPLIED MICROBIOLOGY,2010,51(1):24-29
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