中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 海洋生物  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Zoology
题名: Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum
作者: Zhao, JM ; Qiu, LH ; Ning, XX ; Chen, AQ ; Wu, HF ; Li, CH
通讯作者: chli@yic.ac.cn
关键词: Venerupis philippinarum ; i-type lysozyme ; Tissue expression ; Time-course expression
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2010-01-01
卷: 156, 期:1, 页:56-60
收录类别: sci
部门归属: [Zhao, Jianmin; Chen, Aiqin; Wu, Huifeng; Li, Chenghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qiu, Lihua] Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
项目归属: 海洋生物
摘要: Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. In the present study, an invertebrate type lysozyme (denoted as VpLYZ) was identified from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. The full-length cDNA of VpLYZ consisted of 805 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open-reading frame of 558 bp encoding a polypeptide of 185 amino acids with a calculated molecular mass of 20.87 kD and theoretical pl of 8.44. The high similarity of VpLYZ with other i-type lysozymes from mollusk indicated that VpLYZ should be a new member of i-type lysozyme family. Similar to most i-type lysozymes, VpLYZ possessed all conserved features critical for the fundamental structure and function of i-type lysozymes, such as three catalytic residues (Glu19, Asn72 and Ser75) and i-type specific motif CL(E/L/R/H)C(I/M)C. By semi-quantitative RT-PCR analysis, mRNA transcript of VpLYZ was found to be most abundantly expressed in the tissues of gills, hepatopancreas and haemocytes, weakly expressed in the tissues of muscle, foot and mantle. After clams were challenged by Vibrio anguillarum, the mRNA level of VpLYZ in overall haemocyte population was recorded by quantitative real-time RT-PCR. VpLYZ mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the original level at 96 h. All these results indicated that VpLYZ was involved in the immune response against microbe infection and contributed to the clearance of bacterial pathogens. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
原文出处: 查看原文
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5430
Appears in Collections:海洋生物_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum .pdf(549KB)----开放获取--View Download

Recommended Citation:
Zhao, JM; Qiu, LH; Ning, XX; Chen, AQ; Wu, HF; Li, CH.Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,156(1):56-60
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, JM]'s Articles
[Qiu, LH]'s Articles
[Ning, XX]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, JM]‘s Articles
[Qiu, LH]‘s Articles
[Ning, XX]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum .pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace