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Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum
Zhao, JM; Qiu, LH; Ning, XX; Chen, AQ; Wu, HF; Li, CH; chli@yic.ac.cn
2010
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
卷号156期号:1页码:56-60
摘要Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. In the present study, an invertebrate type lysozyme (denoted as VpLYZ) was identified from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. The full-length cDNA of VpLYZ consisted of 805 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open-reading frame of 558 bp encoding a polypeptide of 185 amino acids with a calculated molecular mass of 20.87 kD and theoretical pl of 8.44. The high similarity of VpLYZ with other i-type lysozymes from mollusk indicated that VpLYZ should be a new member of i-type lysozyme family. Similar to most i-type lysozymes, VpLYZ possessed all conserved features critical for the fundamental structure and function of i-type lysozymes, such as three catalytic residues (Glu19, Asn72 and Ser75) and i-type specific motif CL(E/L/R/H)C(I/M)C. By semi-quantitative RT-PCR analysis, mRNA transcript of VpLYZ was found to be most abundantly expressed in the tissues of gills, hepatopancreas and haemocytes, weakly expressed in the tissues of muscle, foot and mantle. After clams were challenged by Vibrio anguillarum, the mRNA level of VpLYZ in overall haemocyte population was recorded by quantitative real-time RT-PCR. VpLYZ mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the original level at 96 h. All these results indicated that VpLYZ was involved in the immune response against microbe infection and contributed to the clearance of bacterial pathogens. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
部门归属[Zhao, Jianmin; Chen, Aiqin; Wu, Huifeng; Li, Chenghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qiu, Lihua] Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
关键词Venerupis Philippinarum I-type Lysozyme Tissue Expression Time-course Expression
学科领域Biochemistry & Molecular Biology ; Zoology
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收录类别sci
资助项目海洋生物
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5430
专题海洋生物
通讯作者chli@yic.ac.cn
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GB/T 7714
Zhao, JM,Qiu, LH,Ning, XX,et al. Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,156(1):56-60.
APA Zhao, JM.,Qiu, LH.,Ning, XX.,Chen, AQ.,Wu, HF.,...&chli@yic.ac.cn.(2010).Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,156(1),56-60.
MLA Zhao, JM,et al."Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 156.1(2010):56-60.
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