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学科主题: Microbiology
题名: Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
作者: Hu, YF ; Fu, CZ ; Huang, YP ; Yin, YS ; Cheng, G ; Lei, F ; Lu, N ; Li, J ; Ashforth, EJ ; Zhang, LX ; Zhu, BL
通讯作者: zhubaoli@im.ac.cn
关键词: metagenomic library ; marine sediment ; lipolytic ; lipase ; esterase ; fosmid clone
刊名: FEMS MICROBIOLOGY ECOLOGY
发表日期: 2010
卷: 72, 期:2, 页:228-237
收录类别: sci
部门归属: [Hu, Yongfei; Fu, Chengzhang; Huang, Yunpeng; Yin, Yeshi; Cheng, Gong; Lei, Fang; Lu, Na; Li, Jing; Ashforth, Elizabeth Jane; Zhang, Lixin; Zhu, Baoli] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China; [Hu, Yongfei; Fu, Chengzhang; Yin, Yeshi; Cheng, Gong; Lei, Fang] Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China; [Zhang, Lixin] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
项目归属: 海洋生物
摘要: Metagenomic cloning is a powerful tool for the discovery of novel genes and biocatalysts from environmental microorganisms. Based on activity screening of a marine sediment microbial metagenomic library, a total of 19 fosmid clones showing lipolytic activity were identified. After subcloning, 15 different lipolytic genes were obtained; their encoded proteins showed 32-68% amino acid identity with proteins in the database. Multiple sequence alignment and phylogenetic tree analysis demonstrated that most of these predicted proteins are new members of known families of bacterial lipolytic enzymes. However, two proteins, FLS18C and FLS18D, could not be assigned to any known family, thus probably representing a novel family of the bacterial lipolytic enzyme. The activity assay results indicated that most of these lipolytic enzymes showed optimum temperature for hydrolysis at 40-50 degrees C with p-nitrophenol butyrate as a substrate. The lipolytic gene fls18D was overexpressed, and the resulting protein FLS18D was characterized as an alkaline esterase. Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated.
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WOS记录号: WOS:000276490200007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5432
Appears in Collections:海洋生物_期刊论文

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Recommended Citation:
Hu, YF; Fu, CZ; Huang, YP; Yin, YS; Cheng, G; Lei, F; Lu, N; Li, J; Ashforth, EJ; Zhang, LX; Zhu, BL.Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment,FEMS MICROBIOLOGY ECOLOGY,2010,72(2):228-237
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