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学科主题: Biotechnology & Applied Microbiology
题名: Two different electron transfer pathways may involve in azoreduction in Shewanella decolorationis S12
作者: Chen, XJ ; Xu, MY ; Wei, JB ; Sun, GP
通讯作者: xumy@gdim.cn
关键词: Electron transfer pathways ; Azoreduction ; ccmA ; Azo dyes ; Shewanella decolorationis S12
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2010
卷: 86, 期:2, 页:743-751
收录类别: sci
部门归属: [Chen, Xingjuan; Xu, Meiying; Wei, Jinbo; Sun, Guoping] Guangdong Inst Microbiol, Guangzhou 510070, Guangdong, Peoples R China; [Chen, Xingjuan] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Xingjuan; Xu, Meiying; Wei, Jinbo; Sun, Guoping] Guangdong Prov Key Lab Appl Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Guangdong, Peoples R China; [Chen, Xingjuan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
项目归属: 海洋生物
摘要: Electron transfer pathways for azoreduction by S. decolorationis S12 were studied using a mutant S12-22 which had a transposon insertion in ccmA. The results imply that there are two different pathways for electron transport to azo bonds. The colony of S12-22 was whitish and incapable of producing mature c-type cytochromes whose alpha-peak was at 553 nm in the wild type S12. The mutant S12-22 could not use formate as the sole electron donor for azoreduction either in vivo or in vitro, but intact cells of S12-22 were able to reduce azo dyes of low polarity, such as methyl red, when NADH was served as the sole electron donor. Although the highly polar-sulfonated amaranth could not be reduced by intact cells of S12-22, it could be efficiently reduced by cell extracts of the mutant when NADH was provided as the sole electron donor. These results suggest that the mature c-type cytochromes are essential electron mediators for the extracellular azoreduction of intact cells, while the other pathway without the involvement of mature c-type cytochromes, NADH-dependent oxidoreductase-mediated electron transfer pathway can reduce lowly polar sulfonated azo dyes inside the whole cells or highly polar sulfonated azo dyes in the cell extracts without bacterial membrane barriers.
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WOS记录号: WOS:000274707600034
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5436
Appears in Collections:海洋生物_期刊论文

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Chen, XJ; Xu, MY; Wei, JB; Sun, GP.Two different electron transfer pathways may involve in azoreduction in Shewanella decolorationis S12,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,86(2):743-751
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