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学科主题: Biotechnology & Applied Microbiology
题名: Identification of avermectin-high-producing strains by high-throughput screening methods
作者: Gao, H ; Liu, M ; Zhou, XL ; Liu, JT ; Zhuo, Y ; Gou, ZX ; Xu, B ; Zhang, WQ ; Liu, XY ; Luo, AQ ; Zheng, CS ; Chen, XP ; Zhang, LX
通讯作者: Zhanglixin@im.ac.cn
关键词: Avermectin ; High-throughput screening ; Strain improvement ; Miniaturized production
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2010
卷: 85, 期:4, 页:1219-1225
收录类别: sci
部门归属: [Gao, Hong; Liu, Mei; Zhou, Xianlong; Liu, Jintao; Zhuo, Ying; Gou, Zhongxuan; Zhang, Wenquan; Liu, Xiangyang; Luo, Aiqun; Zheng, Chuansen; Zhang, Lixin] Chinese Acad Sci, Inst Microbiol, Beijing 100190, Peoples R China; [Zhang, Lixin] CAS, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Xiaoping; Zhang, Lixin] CAS, Guangzhou Inst Biomed, Guangzhou 510663, Guangdong, Peoples R China; [Chen, Xiaoping; Zhang, Lixin] CAS, Guangzhou Inst Hlth, Guangzhou 510663, Guangdong, Peoples R China; [Liu, Jintao; Zhuo, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Bing] Hebei Veyong Biochem Co LTD, Shijiazhuang 050091, Hebei, Peoples R China
项目归属: 海洋生物
摘要: Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
原文出处: 查看原文
WOS记录号: WOS:000273554900040
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5440
Appears in Collections:海洋生物_期刊论文

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Recommended Citation:
Gao, H; Liu, M; Zhou, XL; Liu, JT; Zhuo, Y; Gou, ZX; Xu, B; Zhang, WQ; Liu, XY; Luo, AQ; Zheng, CS; Chen, XP; Zhang, LX.Identification of avermectin-high-producing strains by high-throughput screening methods,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,85(4):1219-1225
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