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Identification of avermectin-high-producing strains by high-throughput screening methods
Gao, H; Liu, M; Zhou, XL; Liu, JT; Zhuo, Y; Gou, ZX; Xu, B; Zhang, WQ; Liu, XY; Luo, AQ; Zheng, CS; Chen, XP; Zhang, LX; Zhanglixin@im.ac.cn
2010
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号85期号:4页码:1219-1225
摘要Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
部门归属[Gao, Hong; Liu, Mei; Zhou, Xianlong; Liu, Jintao; Zhuo, Ying; Gou, Zhongxuan; Zhang, Wenquan; Liu, Xiangyang; Luo, Aiqun; Zheng, Chuansen; Zhang, Lixin] Chinese Acad Sci, Inst Microbiol, Beijing 100190, Peoples R China; [Zhang, Lixin] CAS, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Xiaoping; Zhang, Lixin] CAS, Guangzhou Inst Biomed, Guangzhou 510663, Guangdong, Peoples R China; [Chen, Xiaoping; Zhang, Lixin] CAS, Guangzhou Inst Hlth, Guangzhou 510663, Guangdong, Peoples R China; [Liu, Jintao; Zhuo, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Bing] Hebei Veyong Biochem Co LTD, Shijiazhuang 050091, Hebei, Peoples R China
关键词Avermectin High-throughput Screening Strain Improvement Miniaturized Production
学科领域Biotechnology & Applied Microbiology
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收录类别sci
资助项目海洋生物
WOS记录号WOS:000273554900040
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5440
专题海洋生物
通讯作者Zhanglixin@im.ac.cn
推荐引用方式
GB/T 7714
Gao, H,Liu, M,Zhou, XL,et al. Identification of avermectin-high-producing strains by high-throughput screening methods[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,85(4):1219-1225.
APA Gao, H.,Liu, M.,Zhou, XL.,Liu, JT.,Zhuo, Y.,...&Zhanglixin@im.ac.cn.(2010).Identification of avermectin-high-producing strains by high-throughput screening methods.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,85(4),1219-1225.
MLA Gao, H,et al."Identification of avermectin-high-producing strains by high-throughput screening methods".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 85.4(2010):1219-1225.
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