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学科主题: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
题名: Chemical characterization and anti-anaemia activity of fish protein hydrolysate from Saurida elongata
作者: Dong, YL ; Sheng, GY ; Fu, JM ; Wen, KW
通讯作者: Shenggy@gig.ac.cn
关键词: anti-anaemia ; enzymatic hydrolysis ; fish protein hydrolysate ; Saurida elongata
刊名: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
发表日期: 2005
卷: 85, 期:12, 页:2033-2039
收录类别: sci
部门归属: Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangdong Key Lab Envionm Protect & Resources Uti, Guangzhou 510640, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Lab Appl Technol Marine Organ, Guangzhou 510301, Peoples R China
项目归属: 广东省应用海洋生物重点实验室
摘要: A low-value sea fish Saurida elongata was hydrolyzed using papain. Proximate composition analysis of the fish protein hydrolysate showed that the hydrolysate contained 84.7% crude protein, 7.1% ash and 3.5% fat. The determination of amino acid content by o-phthaldialdehyde pre-column derivatization reversed-phase high-performance liquid chromatography (RP-HPLC) showed that the hydrolysate contained 20 different amino acids, and the total percentage of eight essential amino acids was as high as 41.5% of the total amino acids, which meant that the hydrolysate could provide a good nitrogen source for human consumption. Peptides were separated by RP-HPLC using a C-18 column into more than 14 fractions. Molecular weight distribution of peptides was determined by size-exclusion HPLC, which indicated that the size of 74.8% of the peptides ranged from 1.0 to 5.0 kDa. Most importantly, the enzymatic hydrolysate from S elongata was shown to contain significant in vivo anti-anaemia activity on experimental anaemia models induced by blood loss or cyclophosphamide damage to hematogenic mechanism. To the best of our knowledge, this was the first time that the description of hydrolysis of S elongata and the indication of anti-anaemia activity in the hydrolysate have been reported. (c) 2005 Society of Chemical Industry.
WOS记录号: WOS:000231633800011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5454
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Recommended Citation:
Dong, YL; Sheng, GY; Fu, JM; Wen, KW.Chemical characterization and anti-anaemia activity of fish protein hydrolysate from Saurida elongata,JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,2005,85(12):2033-2039
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