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学科主题: Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology
题名: Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification
作者: Sun, ZF ; Hu, CQ ; Ren, CH ; Shen, Q
通讯作者: chaoqunhu@sina.com
关键词: loop-mediated isothermal amplification ; IHHNV ; shrimp
刊名: JOURNAL OF VIROLOGICAL METHODS
发表日期: 2006
卷: 131, 期:1, 页:41-46
收录类别: sci
部门归属: Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Province, Guangzhou 510301, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: 广东省应用海洋生物学重点实验室
摘要: A method for nucleic acid amplification, loop-mediated isothermal amplification (LAMP) is a novel, sensitive and rapid technique, which can be applied for disease diagnosis in aquaculture. Using the LAMP method, a highly specific and sensitive diagnostic system for infectious hypodermal and hematopoietic necrosis virus (IHHNV) detection was designed. A set of four primers was designed by targeting the IHHNV genome DNA. By the detection system, target DNA was amplified and visualized on agarose gel within 60 min under isothermal condition at 64 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by addition of SYBR Green I for a naked-eye inspection. The LAMP reaction was also assessed by the white turbidity of magnesium pyrophosphate (a by-product of LAMP) in the tube. The assay had a detection limit of 5-500 copies of DNA template with gel electrophoresis, SYBR Green I and white turbidity with naked-eye inspection. The detection sensitivity of LAMP was 100-fold higher than the PCR. A diagnostic procedure which is rapid and highly sensitive was developed for IHHNV detection. (c) 2005 Elsevier B.V. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000234582800007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5466
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Sun, ZF; Hu, CQ; Ren, CH; Shen, Q.Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification,JOURNAL OF VIROLOGICAL METHODS,2006,131(1):41-46
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文件名: Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification .pdf
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