Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification
Sun, ZF; Hu, CQ; Ren, CH; Shen, Q; chaoqunhu@sina.com
2006
发表期刊JOURNAL OF VIROLOGICAL METHODS
ISSN0166-0934
卷号131期号:1页码:41-46
摘要A method for nucleic acid amplification, loop-mediated isothermal amplification (LAMP) is a novel, sensitive and rapid technique, which can be applied for disease diagnosis in aquaculture. Using the LAMP method, a highly specific and sensitive diagnostic system for infectious hypodermal and hematopoietic necrosis virus (IHHNV) detection was designed. A set of four primers was designed by targeting the IHHNV genome DNA. By the detection system, target DNA was amplified and visualized on agarose gel within 60 min under isothermal condition at 64 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by addition of SYBR Green I for a naked-eye inspection. The LAMP reaction was also assessed by the white turbidity of magnesium pyrophosphate (a by-product of LAMP) in the tube. The assay had a detection limit of 5-500 copies of DNA template with gel electrophoresis, SYBR Green I and white turbidity with naked-eye inspection. The detection sensitivity of LAMP was 100-fold higher than the PCR. A diagnostic procedure which is rapid and highly sensitive was developed for IHHNV detection. (c) 2005 Elsevier B.V. All rights reserved.
部门归属Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Province, Guangzhou 510301, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
关键词Loop-mediated Isothermal Amplification Ihhnv Shrimp
学科领域Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
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收录类别sci
资助项目广东省应用海洋生物学重点实验室
WOS记录号WOS:000234582800007
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被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5466
专题广东省应用海洋生物学重点实验室
通讯作者chaoqunhu@sina.com
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GB/T 7714
Sun, ZF,Hu, CQ,Ren, CH,et al. Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification[J]. JOURNAL OF VIROLOGICAL METHODS,2006,131(1):41-46.
APA Sun, ZF,Hu, CQ,Ren, CH,Shen, Q,&chaoqunhu@sina.com.(2006).Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification.JOURNAL OF VIROLOGICAL METHODS,131(1),41-46.
MLA Sun, ZF,et al."Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification".JOURNAL OF VIROLOGICAL METHODS 131.1(2006):41-46.
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