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学科主题: Zoology
题名: Comparative analysis of 5'-end sequence of the mitochondrial control region of six flatfish species (Pleuronectidae) from the Yellow Sea
作者: Kong, XY ; Yu, JZ ; Zhou, LS ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Pleuronectidae ; mitochondrial control region ; sequence ; TAS ; ETAS ; CSB
刊名: RAFFLES BULLETIN OF ZOOLOGY
发表日期: 2007
页: 111-120
收录类别: sci
部门归属: Ocean Univ Qingdao, China Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China
项目归属: 广东省应用海洋生物学重点实验室
摘要: The 5'-end sequence of the mitochondrial control region (CR) of six flatfishes (Pleuronectidae: Teleostei) from the Yellow Sea (China) and one from the North Sea (Belgium) were determined using polymerase chain reaction (PCR) and direct sequencing. The seven species are the pointhead plaice (Cleisthenes herzensteini), stone flounder (Kareius bicoloratus), variegated flounder (Verasper variegatus), slime flounder (Microstomus achne), finespotted flounder (Pleuronichthys cornutus), yellow striped flounder (Pseudopleuronectes herzensteini) and common dab (Limanda limanda). The sequences obtained were compared and analyzed with those of 10 other flatfish taxa available in databases from the families Paralichthyidae, Pleuronectidae, Scophthalmidae and Soleidae. No tandem repeats were detected at the beginning of this CR sequence of the seven species studied. The structural arrangement of the sequence appeared highly similar among these species, despite their 5'-end portion being more variable and 3'-end portion more conserved. The terminal associated sequence (TAS) motif and its complementary motif (cTAS) were found at the 5'-end portion and the Extended Terminal Associated Sequence (ETAS) of flatfish and its potential secondary structure was described and analyzed. A conserved motif (CM5'd) was also identified in the 5'-end portion through comparisons with previous studies. Both complete and partial conserved sequence blocks (CSB-F and CSB-D) were detected in the central conserved region (CCR) domain. Molecular phylogenetic analysis of the 17 species was conducted based on the 5'-end CR sequence. The phylogenetic relationships agreed well with those based on morphology at the family level and most of those at the genus level. Ten genera of Pleuronectidae grouped firmly in a big branch in the phylogenetic tree generated, supporting their monophyletic origin. Large intra-generic distances between two Limanda species supported the homoplasy observed in the genus Limanda. However, the discrepancy in relationship of Pleuronichthys cornutus to all other species at the molecular and morphological levels in Pleuronectidae, suggested the need for further phylogenetic analysis. The results in this study indicated that 5'-end CR sequence could be reliably used for the analysis and testing of phylogenetic relationships within the Pleuronectiformes.
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WOS记录号: WOS:000244303400015
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5472
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Recommended Citation:
Kong, XY; Yu, JZ; Zhou, LS; Yu, ZN.Comparative analysis of 5'-end sequence of the mitochondrial control region of six flatfish species (Pleuronectidae) from the Yellow Sea,RAFFLES BULLETIN OF ZOOLOGY,2007,():111-120
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