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学科主题: Oceanography
题名: Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii
作者: Yu, ZN ; Wei, XH ; Kong, XY ; Yu, SS
通讯作者: carlzyu@ouc.edu.cn
关键词: Chlamys farreri ; farrer' s scallop ; internal transcribed spacer (ITS-1) ; DNA sequence ; genetic variation
刊名: ACTA OCEANOLOGICA SINICA
发表日期: 2007
卷: 26, 期:1, 页:93-100
收录类别: sci
部门归属: Chinese Acad Sci, S China Inst Oceanol, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China; Ocean Univ Qingdao, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China
项目归属: 广东省应用海洋生物学重点实验室
摘要: Sequence variation of the first internal transcribed spacer of ribosomal DNA (ITS - 1) was examined and its application to the study of genetic variation was explored in four populations of farrer's scallop Chlamys farreri. ITS - I fragments, with a length of about 300 bp, of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P < 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.
原文出处: 查看原文
WOS记录号: WOS:000244330100010
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5474
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Recommended Citation:
Yu, ZN; Wei, XH; Kong, XY; Yu, SS.Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii,ACTA OCEANOLOGICA SINICA,2007,26(1):93-100
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