Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii
Yu, ZN; Wei, XH; Kong, XY; Yu, SS; carlzyu@ouc.edu.cn
2007
发表期刊ACTA OCEANOLOGICA SINICA
ISSN0253-505X
卷号26期号:1页码:93-100
摘要Sequence variation of the first internal transcribed spacer of ribosomal DNA (ITS - 1) was examined and its application to the study of genetic variation was explored in four populations of farrer's scallop Chlamys farreri. ITS - I fragments, with a length of about 300 bp, of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P < 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.
部门归属Chinese Acad Sci, S China Inst Oceanol, Lab Appl Marine Biol, Guangzhou 510301, Peoples R China; Ocean Univ Qingdao, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China
关键词Chlamys Farreri Farrer' s Scallop Internal Transcribed Spacer (Its-1) Dna Sequence Genetic Variation
学科领域Oceanography
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收录类别sci
资助项目广东省应用海洋生物学重点实验室
WOS记录号WOS:000244330100010
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5474
专题广东省应用海洋生物学重点实验室
通讯作者carlzyu@ouc.edu.cn
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GB/T 7714
Yu, ZN,Wei, XH,Kong, XY,et al. Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii[J]. ACTA OCEANOLOGICA SINICA,2007,26(1):93-100.
APA Yu, ZN,Wei, XH,Kong, XY,Yu, SS,&carlzyu@ouc.edu.cn.(2007).Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii.ACTA OCEANOLOGICA SINICA,26(1),93-100.
MLA Yu, ZN,et al."Application of the first internal transcribed spacer (ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer's scallop Chlamys farreii".ACTA OCEANOLOGICA SINICA 26.1(2007):93-100.
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