Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification
Ren, CH; Hu, CQ; Luo, P; Wang, QB; cqhu@scsio.ac.cn
2009
发表期刊MICROBIOLOGICAL RESEARCH
ISSN0944-5013
卷号164期号:5页码:514-521
摘要Vibrio vulnificus is a serious bacterial. pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of V. vulnificus. The LAMP reaction was processed in a heat block at 65 degrees C for 60 min. The amplification products were detected by visual inspection using SYBR Green 1, as welt as by electrophoresis on agarose gets. Our results showed that the LAMP reaction was highly specific to V. vulnificus. This method was 10-fold more sensitive than conventional PCR. In conclusion, the LAMP assay was extremely rapid, simple, cost-effective, sensitive and specific for the rapid identification of V. vulnificus. (C) 2008 Elsevier GmbH. All rights reserved.
部门归属[Hu, Chao-Qun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
关键词Identification Vibrio Vulnificus Lamp
学科领域Microbiology
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收录类别sci
资助项目广东省应用海洋药物重点实验室
WOS记录号WOS:000271104500002
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5476
专题广东省应用海洋生物学重点实验室
通讯作者cqhu@scsio.ac.cn
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Ren, CH,Hu, CQ,Luo, P,et al. Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification[J]. MICROBIOLOGICAL RESEARCH,2009,164(5):514-521.
APA Ren, CH,Hu, CQ,Luo, P,Wang, QB,&cqhu@scsio.ac.cn.(2009).Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification.MICROBIOLOGICAL RESEARCH,164(5),514-521.
MLA Ren, CH,et al."Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification".MICROBIOLOGICAL RESEARCH 164.5(2009):514-521.
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