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学科主题: Microbiology
题名: Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification
作者: Ren, CH ; Hu, CQ ; Luo, P ; Wang, QB
通讯作者: cqhu@scsio.ac.cn
关键词: Identification ; Vibrio vulnificus ; LAMP
刊名: MICROBIOLOGICAL RESEARCH
发表日期: 2009
卷: 164, 期:5, 页:514-521
收录类别: sci
部门归属: [Hu, Chao-Qun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: 广东省应用海洋药物重点实验室
摘要: Vibrio vulnificus is a serious bacterial. pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of V. vulnificus. The LAMP reaction was processed in a heat block at 65 degrees C for 60 min. The amplification products were detected by visual inspection using SYBR Green 1, as welt as by electrophoresis on agarose gets. Our results showed that the LAMP reaction was highly specific to V. vulnificus. This method was 10-fold more sensitive than conventional PCR. In conclusion, the LAMP assay was extremely rapid, simple, cost-effective, sensitive and specific for the rapid identification of V. vulnificus. (C) 2008 Elsevier GmbH. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000271104500002
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5476
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Recommended Citation:
Ren, CH; Hu, CQ; Luo, P; Wang, QB.Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification,MICROBIOLOGICAL RESEARCH,2009,164(5):514-521
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