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SCSIO OpenIR  > 广东省应用海洋生物学重点实验室  > 期刊论文
学科主题: Gastroenterology & Hepatology
题名: Up-regulation of PIK3CA promotes metastasis in gastric carcinoma
作者: Liu, JF ; Zhou, XK ; Chen, JH ; Yi, G ; Chen, HG ; Ba, MC ; Lin, SQ ; Qi, YC
通讯作者: zxkstar@126.com
关键词: PIK3CA ; Phosphatidylinositol 3-kinase/Akt pathway ; Metastasis ; Gastric cancer ; Akt
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2010
卷: 16, 期:39, 页:4986-4991
收录类别: sci
部门归属: [Liu, Ji-Fang; Zhou, Xin-Ke; Yi, Gao; Lin, Sheng-Qu; Qi, Yan-Chao] Guangzhou Med Coll, Affiliated Tumor Hosp, Canc Res Inst, Guangzhou 510095, Guangdong, Peoples R China; [Chen, Jin-Hui] CAS, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Jin-Hui] Chinese Acad Sci, So China Sea Inst Oceanol, Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, He-Ge] Univ Maryland, Dept Pharmacol & Expt Therapeut, Catonsville, MD 21228 USA; [Ba, Ming-Chen] Guangzhou Med Coll, Affiliated Tumor Hosp, Dept Abdominal Surg, Sect 2, Guangzhou 510095, Guangdong, Peoples R China
项目归属: 广东省应用海洋生物学重点实验室
摘要: AIM: To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway. METHODS: mRNA and protein levels of PIK3CA were assessed, respectively, by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa, primary foci and lymph node and distant metastasis of gastric cancer. Akt and phosphorylated Akt protein were also examined by Western blotting in these tissues, in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling pathway. RESULTS: PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher, respectively, than that in the corresponding normal gastric mucosa and primary gastric cancer tissues (P < 0.05), while no statistical significance was found compared with distant metastasis. Immunohistochemically, PIK3CA protein expression was discovered in 7 (35%) specimens of 20 primary foci vs 10 (67%) of 15 of lymph node metastasis or 11(61%) of 18 of distant metastasis (35% vs 67%, P = 0.015; 35% vs 61%, P = 0.044). With the increased level of PIK3CA expression, the total Akt protein expression remained almost unchanged, but p-Akt protein was upregulated markedly. CONCLUSION: Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric cancer. Up-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling. (C) 2010 Baishideng. All rights reserved.
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WOS记录号: WOS:000283396600015
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5480
Appears in Collections:广东省应用海洋生物学重点实验室_期刊论文

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Liu, JF; Zhou, XK; Chen, JH; Yi, G; Chen, HG; Ba, MC; Lin, SQ; Qi, YC.Up-regulation of PIK3CA promotes metastasis in gastric carcinoma,WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(39):4986-4991
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