Up-regulation of PIK3CA promotes metastasis in gastric carcinoma
Liu, JF; Zhou, XK; Chen, JH; Yi, G; Chen, HG; Ba, MC; Lin, SQ; Qi, YC; zxkstar@126.com
2010
发表期刊WORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
卷号16期号:39页码:4986-4991
摘要AIM: To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway. METHODS: mRNA and protein levels of PIK3CA were assessed, respectively, by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa, primary foci and lymph node and distant metastasis of gastric cancer. Akt and phosphorylated Akt protein were also examined by Western blotting in these tissues, in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling pathway. RESULTS: PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher, respectively, than that in the corresponding normal gastric mucosa and primary gastric cancer tissues (P < 0.05), while no statistical significance was found compared with distant metastasis. Immunohistochemically, PIK3CA protein expression was discovered in 7 (35%) specimens of 20 primary foci vs 10 (67%) of 15 of lymph node metastasis or 11(61%) of 18 of distant metastasis (35% vs 67%, P = 0.015; 35% vs 61%, P = 0.044). With the increased level of PIK3CA expression, the total Akt protein expression remained almost unchanged, but p-Akt protein was upregulated markedly. CONCLUSION: Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric cancer. Up-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling. (C) 2010 Baishideng. All rights reserved.
部门归属[Liu, Ji-Fang; Zhou, Xin-Ke; Yi, Gao; Lin, Sheng-Qu; Qi, Yan-Chao] Guangzhou Med Coll, Affiliated Tumor Hosp, Canc Res Inst, Guangzhou 510095, Guangdong, Peoples R China; [Chen, Jin-Hui] CAS, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Chen, Jin-Hui] Chinese Acad Sci, So China Sea Inst Oceanol, Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Chen, He-Ge] Univ Maryland, Dept Pharmacol & Expt Therapeut, Catonsville, MD 21228 USA; [Ba, Ming-Chen] Guangzhou Med Coll, Affiliated Tumor Hosp, Dept Abdominal Surg, Sect 2, Guangzhou 510095, Guangdong, Peoples R China
关键词Pik3ca Phosphatidylinositol 3-kinase/akt Pathway Metastasis Gastric Cancer Akt
学科领域Gastroenterology & Hepatology
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收录类别sci
资助项目广东省应用海洋生物学重点实验室
WOS记录号WOS:000283396600015
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被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5480
专题广东省应用海洋生物学重点实验室
通讯作者zxkstar@126.com
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GB/T 7714
Liu, JF,Zhou, XK,Chen, JH,et al. Up-regulation of PIK3CA promotes metastasis in gastric carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(39):4986-4991.
APA Liu, JF.,Zhou, XK.,Chen, JH.,Yi, G.,Chen, HG.,...&zxkstar@126.com.(2010).Up-regulation of PIK3CA promotes metastasis in gastric carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,16(39),4986-4991.
MLA Liu, JF,et al."Up-regulation of PIK3CA promotes metastasis in gastric carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 16.39(2010):4986-4991.
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