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学科主题: Marine & Freshwater Biology
题名: Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis
作者: Shao, P ; Chen, YQ ; Zhou, H ; Yuan, J ; Qu, LH ; Zhao, D ; Lin, YS
通讯作者: lsbrc04@zsu.edu.cn
刊名: MARINE BIOLOGY
发表日期: 2004
卷: 144, 期:2, 页:215-224
收录类别: sci
部门归属: Zhongshan Univ, Biotechnol Res Ctr, Key Lab Gene Engn Educ Minist, Guangzhou 510275, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Lab Trop Marine Environm Dynam, Beijing 100864, Peoples R China
项目归属: LED
摘要: The internal transcribed spacer (ITS) regions and 5.8S rRNA genes from several strains of toxic Gymnodiniaceae were sequenced and subjected to phylogenetic analysis with other Gymnodiniaceae species. Sequence comparisons showed that high sequence divergence existed in Gymnodiniaceae, especially in the genus Gymnodinium. The amplicons of the ITS regions from Amphidinium and Gyrodinium species were 438-439 and 604-605 bp, respectively, and those of the Gymnodinium species ranged from 575 to 615 bp. The mean distance value within Gymnodinium, calculated from the ITS sequence, was 0.68827 (range: 0-0.92323), 0.11342 for Amphidinium (range: 0.00467-0.17120) and 0.2005 for Karenia (range: 0.00521-0.29971). Low distance values were found within the species Gyrodinium instriatum (<0.01) and Karlodinium micrum (<0.02). Amphidinium remarkably had a shorter ITS than did other genera in Gymnodiniaceae, this implied that Amphidinium might be distant from the other Gymnodiniaceae and supported Saunders' opinion that the taxonomy of Amphidinium needs to be reevaluated. Largely congruent phylogenetic trees were produced by the maximum-parsimony method (PAUP), maximum-likelihood method (PAUP) and Bayesian inference (MrBayes), whereas the three analyses showed that the genera Gymnodinium and Karenia are unresolved groups in phylogeny. Minor sequence divergence was found within the different clones of Amphidinium carterae, suggesting that the ITS regions are suitable as genus- and species-specific oligonucleotide probes to rapidly detect and identify the red tide-forming algal species.
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WOS记录号: WOS:000188496200002
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5671
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Shao, P; Chen, YQ; Zhou, H; Yuan, J; Qu, LH; Zhao, D; Lin, YS.Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis,MARINE BIOLOGY,2004,144(2):215-224
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