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Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection
He, HZ; Li, HB; Chen, F; sfchen@hkusua.hku.hk
2005
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume383Issue:5Pages:875-879
AbstractA highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B-1. Vitamin B-1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mmx4.6 mm ID, 5 mu m) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00x10(-10) mol L-1 to 5.00x10(-7) mol L-1 for vitamin B-1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0x10(-10) mol L-1. The method was successfully used for determination of vitamin B-1 at pg mL(-1) levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%.
DepartmentUniv Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China
KeywordVitamin B-1 High-performance Liquid Chromatography Solid-phase Extraction Fermentation Media Seawater
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
Indexed Bysci
Funding ProjectLED
WOS IDWOS:000233874900026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/5683
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorsfchen@hkusua.hku.hk
Recommended Citation
GB/T 7714
He, HZ,Li, HB,Chen, F,et al. Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(5):875-879.
APA He, HZ,Li, HB,Chen, F,&sfchen@hkusua.hku.hk.(2005).Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,383(5),875-879.
MLA He, HZ,et al."Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 383.5(2005):875-879.
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