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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection
作者: He, HZ ; Li, HB ; Chen, F
通讯作者: sfchen@hkusua.hku.hk
关键词: vitamin B-1 ; high-performance liquid chromatography ; solid-phase extraction ; fermentation media ; seawater
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2005
卷: 383, 期:5, 页:875-879
收录类别: sci
部门归属: Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China
项目归属: LED
摘要: A highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B-1. Vitamin B-1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mmx4.6 mm ID, 5 mu m) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00x10(-10) mol L-1 to 5.00x10(-7) mol L-1 for vitamin B-1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0x10(-10) mol L-1. The method was successfully used for determination of vitamin B-1 at pg mL(-1) levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%.
WOS记录号: WOS:000233874900026
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5683
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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He, HZ; Li, HB; Chen, F.Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(5):875-879
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文件名: Determination of vitamin B1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection .pdf
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