Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection
He, HZ; Li, HB; Jiang, Y; Chen, F; sfchen@hkusua.hku.hk
2005
发表期刊ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
卷号383期号:6页码:1014-1017
摘要A novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a mu Bondapak NH2 column (300 mm x 3.9 mm, 10 mu m) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min(-1)) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL(-1), respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL(-1), respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88 +/- 2% to 107 +/- 4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase.
部门归属Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China; Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
关键词Paralytic Shellfish Poisoning Toxins High Performance Liquid Chromatography Fluorescence Detection Microalgae
学科领域Biochemical Research Methods ; Chemistry, Analytical
收录类别sci
资助项目LED
WOS记录号WOS:000233875200017
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5685
专题热带海洋环境国家重点实验室(LTO)
通讯作者sfchen@hkusua.hku.hk
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GB/T 7714
He, HZ,Li, HB,Jiang, Y,et al. Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(6):1014-1017.
APA He, HZ,Li, HB,Jiang, Y,Chen, F,&sfchen@hkusua.hku.hk.(2005).Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,383(6),1014-1017.
MLA He, HZ,et al."Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 383.6(2005):1014-1017.
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