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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection
作者: He, HZ ; Li, HB ; Jiang, Y ; Chen, F
通讯作者: sfchen@hkusua.hku.hk
关键词: paralytic shellfish poisoning toxins ; high performance liquid chromatography ; fluorescence detection ; microalgae
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2005
卷: 383, 期:6, 页:1014-1017
收录类别: sci
部门归属: Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Peoples R China; Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
项目归属: LED
摘要: A novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a mu Bondapak NH2 column (300 mm x 3.9 mm, 10 mu m) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min(-1)) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL(-1), respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL(-1), respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88 +/- 2% to 107 +/- 4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase.
WOS记录号: WOS:000233875200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5685
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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He, HZ; Li, HB; Jiang, Y; Chen, F.Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(6):1014-1017
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文件名: Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection .pdf
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