Biochemical pathway and degradation of phthalate ester isomers by bacteria
Gu, JD; Li, J; Wang, Y; jdgu@hkucc.hku.hk
2005
发表期刊WATER SCIENCE AND TECHNOLOGY
ISSN0273-1223
卷号52期号:8页码:241-248
摘要Degradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading DMI alone. In co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (MMI) ester and isophthalic acid (IPA) before the aromatic ring opening. Using DMI as the sole carbon and energy source, Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr degraded DMI through the biochemical cooperation. The initial hydrolytic reaction of the ester bond was by K oxytoca Sc and the next step of transformation was by M. mesophilicum Sr, and IPA was degraded by both of them. In another investigation, a novel bacterium, strain MPsc, was isolated for degradation of dimethyl phthalate ester (DMPE) also from the mangrove sediment. On the basis of phenotypic, biochemical and 16S rDNA gene sequence analyses, the strain MPsc should be considered as a new bacterium at the genus level (8% differences). This strain, together with a Rhodococcus zopfii isolated from the same mangrove sediment, was able to degrade DMPE aerobically. The consortium consisting of the two species degraded 450 mg/l DMPE within 3 days as the sole source of carbon and energy, but none of the individual species alone was able to transform DMPE. Furthermore, the biochemical degradation pathway proceeded through monomethyl phthalate (MMP), phthalic acid (PA) and then protocatechuate before aromatic ring cleavage. Our results suggest that degradation of complex organic compounds including DMI and DMPE may be carried out by several members of microorganisms working together in the natural environments.
部门归属Univ Hong Kong, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam, Environm & Mol Microbiol, Guangzhou 510301, Peoples R China; Univ Hong Kong, Dept Ecol & Biodivers, Lab Environm Toxicol, Hong Kong, Hong Kong, Peoples R China
关键词Biochemical Cooperation Degradation Phthalate Ester Plasticizer Wetland
学科领域Engineering, Environmental ; Environmental Sciences ; Water Resources
收录类别sci
资助项目LED
WOS记录号WOS:000233644300029
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5687
专题热带海洋环境国家重点实验室(LTO)
通讯作者jdgu@hkucc.hku.hk
推荐引用方式
GB/T 7714
Gu, JD,Li, J,Wang, Y,et al. Biochemical pathway and degradation of phthalate ester isomers by bacteria[J]. WATER SCIENCE AND TECHNOLOGY,2005,52(8):241-248.
APA Gu, JD,Li, J,Wang, Y,&jdgu@hkucc.hku.hk.(2005).Biochemical pathway and degradation of phthalate ester isomers by bacteria.WATER SCIENCE AND TECHNOLOGY,52(8),241-248.
MLA Gu, JD,et al."Biochemical pathway and degradation of phthalate ester isomers by bacteria".WATER SCIENCE AND TECHNOLOGY 52.8(2005):241-248.
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