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学科主题: Engineering, Environmental; Polymer Science
题名: Comparing the growth characteristics of three bacteria involved in degrading rubbers
作者: Gu, JD ; Pan, L
通讯作者: jdgu@hkucc.hku.hk
关键词: Bradford assay ; Coomassie Brilliant Blue-G250 ; bacterial growth ; polymer deterioration
刊名: JOURNAL OF POLYMERS AND THE ENVIRONMENT
发表日期: 2006
卷: 14, 期:3, 页:273-279
收录类别: sci
部门归属: Univ Hong Kong, Dept Ecol & Biodivers, Lab Environm Microbiol & Toxicol, Hong Kong, Hong Kong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Peoples R China
项目归属: LED
摘要: Three bacteria isolated for degradation of rubbers were compared for their growth characteristics derived from the Bradford protein assay and turbidity (optical density, OD) measurement. Both Alcaligenes xylosoxidans T2 and Pseudomonas aeruginosa GP10 were fast-growing bacteria while Nocardia corynebacterioides S3 was a slow grower utilizing rubber as the sole source of carbon and energy, but the extent of degradation was lower by the formers than the latter. A. xylosoxidans T2, P. aeruginosa GP10 and N. corynebacterioides S3 showed a typical sigmoidal growth pattern based on binding of Coomassie Brilliant Blue G250 to bacterial proteins and spectrophotometrical measurement at 600 nm. Both assays showed similar growth characteristics for all three bacteria in this study. Degradation of rubber was more pronounced by N. corynebacterioides S3 than either A. xylosoxidans T2 or P. aeruginosa GP10 during 70 days of incubation. Our results suggest that slow-growing bacteria may play a much greater role in degrading polymeric materials than was previously believed.
原文出处: 查看原文
WOS记录号: WOS:000242286100008
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5767
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Gu, JD; Pan, L.Comparing the growth characteristics of three bacteria involved in degrading rubbers,JOURNAL OF POLYMERS AND THE ENVIRONMENT,2006,14(3):273-279
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