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Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs
Yin, B; Huang, LM; Gu, JD; jdgu@hkucc.hku.hk
2006
Source PublicationWATER AIR AND SOIL POLLUTION
ISSN0049-6979
Volume176Issue:40547Pages:185-199
AbstractIndolic compounds are N-heterocyclic aromatic chemicals and have been detected at contaminated sites. Biodegradation of 1-methylindole (1MI) and 3-methylindole (3MI) was investigated initially using enrichment cultures with mangrove sediment obtained from Mai Po Nature Reserve of Hong Kong and subsequently with a pure culture of Pseudomonas aeruginosa Gs confirmed with 16S rRNA gene. At 2.0 mM, 1MI and 3MI were degraded in 4 and 3 days, respectively, by the respective 1MI- and 3MI-degrading enrichment cultures. When substrate concentrations were increased to 3.0,mM and 3.5,mM, slower degradation of 1MI and 3MI was observed indicating inhibitory effects from the substrates, possibly due to toxicity. In addition, no colony of bacteria could be observed on the agar plates amended with 3.5,mM 1MI or 4.0,mM 3MI, indicating that 1MI was more toxic than 3MI. Pseudomonas aeruginosa Gs, isolated from the enrichment culture, effectively utilized both substrates as the sole source of carbon and energy. Complete degradation of 1MI and 3MI was achieved after more than 40 days and 24 days, respectively, at an initial concentration of 2,mM in the culture. Effects of initial substrate concentration, pH and salinity on degradation of 1MI and 3MI by P. aeruginosa Gs were also studied in batch culture. The optimum pH and salinity for degrading both substrates by P. aeruginosa Gs was 7.0 and 5 parts per thousand, respectively. Biodegradation kinetics of 1MI and 3MI by P. aeruginosa Gs could be described using a first-order kinetic model. Our results suggest that both 1MI and 3MI are biodegradable in the mangrove environment and that toxicity of 1MI could be a potential factor limiting the removal of the chemical in the environment by microorganisms.
DepartmentChinese Acad Sci, Key Lab Trop Marine Environm Dynam LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Univ Hong Kong, Lab Environm Toxicol, Dept Ecol & Biodivers, Hong Kong, Hong Kong, Peoples R China; Univ Hong Kong, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China
KeywordDegradation 1-methylindole 3-methylindole Mangrove Sediment Pseudomonas Aeruginosa Gs First-order Kinetic Model
Subject AreaEnvironmental Sciences ; Meteorology & Atmospheric Sciences ; Water Resources
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Indexed Bysci
Funding ProjectLED
WOS IDWOS:000240318000012
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/5773
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorjdgu@hkucc.hku.hk
Recommended Citation
GB/T 7714
Yin, B,Huang, LM,Gu, JD,et al. Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs[J]. WATER AIR AND SOIL POLLUTION,2006,176(40547):185-199.
APA Yin, B,Huang, LM,Gu, JD,&jdgu@hkucc.hku.hk.(2006).Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs.WATER AIR AND SOIL POLLUTION,176(40547),185-199.
MLA Yin, B,et al."Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs".WATER AIR AND SOIL POLLUTION 176.40547(2006):185-199.
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